Pharmaceutical uses and synthesis of nicotinanilide-N-oxides

ABSTRACT

Disclosed are nicotinanilide-N-oxide compounds, methods for their production, pharmaceutical compositions which include these compounds, and methods for their use in various therapies.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. patent application Ser. No.10/015,861 filed Dec. 12, 2001 (now pending); which claims the benefitof U.S. Provisional Patent Application No. 60/258,730, filed Dec. 29,2000, where these applications are incorporated herein by reference intheir entireties.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to nicotinanilide N-oxide compounds usefulas pharmaceutical agents, to methods for their production, topharmaceutical compositions which include these compounds, and tomethods for their use in various therapies.

2. Description of the Related Art

Chemotactic cytokines (chemokines) are a class of potent inflammatorymediators that have the potential to attract specific subsets ofleukocytes to sites of inflammation. Chemokines are typicallylow-molecular-mass (7-9 kd) proteins that can be divided into foursubfamilies (CCC or β-subfamily, CXC or α-subfamily, CX₃C) and arecategorized by their primary amino acid structure. The CXC subfamily ischaracterized by the two conserved Cys residues (C) near the N-terminusand separated by an amino acid (X). Some of the CXC chemokines, of whichIL-8 and GRO-α are representative, belong further to the ELR+ subfamily(Glu-Leu-Arg) and are important in the recruitment and activation ofneutrophils via the CXCR1 and CXCR2 receptors.

The interaction of chemokines with specific cell populations is mediatedby G-protein-coupled seven-transmembrane receptors (7TMR). Chemokinereceptors can be classified into four groups (CR, CCR, CXCR, CX3CR)based upon their primary amino acid sequence. The CXCR1 receptor bindswith high affinity to IL-8 and low affinity to NAP-2, ENA-78 (epithelialcell-derived neutrophil-activating factor), GRO-α, -β, and -γ, whereas,CXCR2 binds with high affinity to all of the mentioned CXC chemokines.Both CXCR1 and CXCR2 receptors are found primarily on neutrophils and asubset of T-cells. W. Holmes et al., Science 253:1278 (1991); P. Murphyet al., Science 253:1280 (1991); A. Chuntharapai et al., J. Immunol.153:5682 (1994); L. Xu et al., J. Leukocyte Biol. 57:335 (1995).

CXCR1 and CXCR2 have been shown to mediate the responses to CXCchemokines in neutrophils (polymorphonuclear neutrophils; PMN) and areessential to the acute inflammatory response. P. Grob et al., J. Biol.Chem. 265:8311 (1990); J. Besemer et al., J. Biol. Chem. 264:17, 409(1989); A. Samanta et al., J. Exp. Med. 169:1185 (1989); W. Holmes etal., Science 253:1280 (1991); P. Murphy et al., Science 253:1280 (1991).Although both receptors are involved in neutrophil chemotaxis, in vitrostudies using human neutrophils have shown inconclusively if chemotaxisis mediated by one or both receptors. IL-8 induced chemotaxis studiesusing anti-receptor monoclonal antibodies in CXCR1 and CXCR2 cell lineshave led to conflicting reports. J. Quan et al., Biochem. Biophys. Res.Commun. 219:405 (1996); A. Chuntharapai et al., J. Immunol. 155:2587(1995); M. Hammond et al., J. Immunol. 155:1428 (1995). There is alsoevidence to indicate that the transendothelial migration of CLA+ T-cellsis a CXCR2 mediated event. L. Santamaria-Babi et al., Eur. J. Immuno.26:2056 (1996).

The role, in inflammatory disorders, of neutrophil chemotaxis mediatedby the CXCR1 and CXCR2 receptors is generally accepted. It has beenreported that neutrophils are implicated in the pathogenesis of theacute respiratory distress syndrome (ARDS) in patients with sepsis. J.Repine et al., Am. Rev. Respir. Dis. 144:251 (1991). A causal role ofPMNs in the lung injury associated with trauma is also widely accepted.G. Goldman et al., Ann. Surg. 212:513 (1990); S. Linas et al., Am. J.Physiol. 255:F728 (1988); R Simpson et al., Prog. Clin. Biol. Res.388:265 (1994); S. Donnelly, Arch. Emerg. Med. 10:108 (1993); S.Donnelly, Resuscitation 28:87 (1994). For example, sepsis-related ARDSpatients have increased levels of IL-8, ENA-78, ad GRO-α in theirbronchoalveolar lavage fluids. R. Goodman et al., Am. J. Respir. Crit.Care Med. 154:602 (1996); J. Villard, Am. J. Respir. Crit. Care Med.152:1549 (1995). Additionally, it has been demonstrated that CXCR1functions as the single dominant CXC chemokine receptor for neutrophilchemotaxis in patients with sepsis. C. Cummings, J. Immunol. 162:2341(1999).

High levels of IL-8 and tissue neutrophil infiltration have beenobserved in the synovial tissues of rheumatoid arthritis patients (H.Endo, Lymphokine Cytokine Res. 10:245 (1991)). Evidence has beenpresented that GRO-α and IL-8 are important mediators involved in therecruitment of neutrophils in the early and late phase oflipopolysaccharide-induced (LPS) rabbit arthritis. A. Matsukawa et al.,Lab. Invest. 79:591 (1999). The murine CXCR2 receptor has also shown tobe necessary for neutrophilic inflammation in a mouse model of goutysynovitis. R. Terkeltaub et al., Arthritis. Rheum. 41:900 (1998).

CXC chemokines have attracted attention as being important in thedevelopment of atherosclerosis. R. Terkeltaub et al., Curr. Opin.Lipidol. 9:397 (1998). The role of CXCR1 and CXCR2 ligands on monocytefunction in atherosclerosis in rabbits was published by D. Schwartz etal., J. Clin. Invest. 94:1968 (1994). Knockout mice that lacked CXCŔ2expression had diminished lesion size. W. Boisvert et al., J. Clin.Invest. 101:353 (1998).

The involvement of the CXCR2 receptor in the pathological inflammatoryresponse elicited by central nervous system (CNS) cells as related toAlzheimer's disease is also gaining significant attention. M. Xia etal., J. Neurovirol. 5:32 (1999). Reports have focused on theupregulation of CXCR2 expression on dystrophic neurites of senileplaques. M Xia et al., Am. J. Pathol. 150:1267 (1997); R. Horuk et al.,J. Immunol. 158:2882 (1997).

High levels of IL-8 and neutrophil infiltration have been observed inthe pathogenesis of a number of other disease indications. This includesulcerative colitis (Y. Mahida, Clin. Sci. 82:273 (1992); R. Izzo, Am. J.Gastroenterol 87:1447 (1992)) and psoriasis (R. Gillitzer et al., J.Invest. Dermatol. 107:778 (1996); T. Kojima., J. Invest. Dermatol101:767 (1993)). CXCR1 and CXCR2 chemokines and their roles in tumorgrowth and metastasis have been reviewed. J. Wang, J. Immunol. Meth.220:1 (1998).

To date, a limited number of CXCR1 and CXCR2 antagonists have beenreported. It was reported that a bis-aryl urea was able selectivelyinhibit CXCR2 and prevent neutrophil migration and chemotaxis in arabbit model. J. White, J. Biol. Chem. 273:10095 (1998). Other CXCR1 andCXCR2 receptor antagonists have focused on NH2-terminal truncations andmodifications of IL-8, GRO-α, and ELR motif. S. Jones et al., J. Biol.Chem. 272:16166. Murine neutrophil recruitment in vivo could also beinhibited via CXCR2 receptor blocking using a truncated human GRO-αanalog. There are currently no CXCR1 or CXCR2 receptor antagonist basedtherapies widely available.

There is a continued need for the treatment of diseases mediated by theCXCR1 and CXCR2 receptors. Small molecule antagonists of CXC receptorsand their ligands such as GRO-α and IL-8 would be useful in the controlof harmful inflammatory processes as well as important tools for theinvestigation of receptor-ligand interactions.

BRIEF SUMMARY OF THE INVENTION

The present invention relates to novel nicotinanilide N-oxide compoundsuseful as pharmaceutical agents, to methods for their production, topharmaceutical compositions which include these compounds and apharmaceutical carrier, and to pharmaceutical methods of treatment. Thecompounds of the present invention are G-protein-coupled, seventransmembrane domain (7TM) receptor antagonists. In particular, thecompounds of the invention are useful for the prophylaxis and treatmentof diseases or conditions involving inflammation due to neutrophilchemotaxis mediated via the CXCR1 and CXCR2 receptors. This inventionalso relates to intermediates and processes useful in the preparation ofsuch compounds.

In one aspect, the present invention provides a compound having thestructure (I):

-   -   and optical isomers, diastereomers, enantiomers and        pharmaceutically acceptable salts thereof, wherein R¹ is        selected from R⁵ and R⁵-(C₁-C₆heteroalkylene)- where R⁵ is        selected from hydrogen, halogen, alkyl, heteroalkyl, aryl,        heteroaryl, carbocycle aliphatic ring and heterocycle aliphatic        ring, amino or hydroxy; R² and R³ are independently hydrogen,        alkyl, heteroalkyl, aryl, aryl(akylene), heteroaryl,        heteroaryl(alkylene), carbocycle, carbocycle(alkylene),        heterocycle, and heterocycle(alkylene); each occurrence of R⁴ is        independently selected from halogen, alkyl, heteroalkyl, aryl,        heteroaryl, carbocycle aliphatic ring and heterocycle aliphatic        ring, amino or hydroxy; and n is 0, 1, 2 or 3.

In various aspects, the present invention provides compounds ofstructure (I) as defined above, however: n is 0; or n is 1; or R² is H;or n is 0 and R² is H; or n is 1 and R² is H.

In addition, in any of the above-described aspects, the presentinvention provides compounds wherein R¹ is R⁵—SO₂— and R⁵ is selectedfrom alkyl, heteroalkyl, aryl, carbocycle, aryl(alkylene), andcarbocycle(alkylene); or R¹ is R⁵—SO₂— and R⁵ is selected from alkyl,heteroalkyl, aryl, carbocycle, aryl(alkylene), and carbocycle(alkylene)where alkyl is C₁-C₁₀alkyl, heteroalkyl is C₁-C₁₀alkyl with 1, 2 or 3heteroatoms selected from N, O and S, aryl is phenyl, substitutedphenyl, naphthyl or substituted naphthyl, carbocycle is C₃-C₈carbocycle,and alkylene is C₁-C₁₀alkylene; or R¹ is R⁵—SO₂— and R⁵ is selected fromalkyl, heteroalkyl, aryl, carbocycle, aryl(alkylene), andcarbocycle(alkylene) such that R¹ is selected from (C₁-C₆alkyl)SO₂—,PhSO₂—, fluorinatedphenylSO₂—, PhCH₂SO₂—, cyclopentylSO₂—,m-carboxyphenylSO₂—, m-methylphenylSO₂—, and HOOC—(C₁-C₄alkylene)SO₂—.

In addition, in any of the above-described aspects, unless otherwiseinconsistent therewith, the present invention provides compounds ofstructure (I) wherein R¹ is selected from halogen, amino,hydrocarbylamino, dihydrocarbylamino, hydrocarbyloxy, hydrocarbylthio,heterocyclyl, (heteroalkyl)amino, and (heteroaryl)amino; optionally, R¹is selected from amino, (C₁-C₆alkyl)(C₁-C₆alkyl)amino, PhNH—, PhCH₂NH—,

and HOCH₂CH₂NH—; optionally, R¹ is selected from halide and(C₁-C₆alkyl)S—; optionally R¹ is chloride.

In addition, in any of the above-described aspects, unless otherwiseinconsistent therewith, the present invention provides compounds ofstructure (I) wherein R³ is selected from aryl, aryl(alkylene),heteroaryl, and heteroaryl(alkylene); optionally R³ is aryl.

In another aspect, the present invention provides a compound havingstructure (II)

In addition, in any of the above-described aspects, unless otherwiseinconsistent therewith, the present invention provides compounds ofstructure (I) wherein R³ is benzyl or phenyl, the benzyl or phenylhaving 0, 1, 2, 3 or 4 substituents selected from alkoxy,alkoxycarbonyl, alkyl, alkylamido, alkylcarbonyl, amido, benzyloptionally substituted with halogen, benzyloxy, carboxy, cyano,dialkylamido, haloalkyl, haloalkyloxy, halogen, hydroxy, nitro,oxoalkyl, phenyl optionally substituted with halogen, thioalkyl,thiocyanate, and thiohaloalkyl.

In addition, in any of the above-described aspects, unless otherwiseinconsistent therewith, the present invention provides compounds ofstructure (I) wherein R³ is selected from cycloalkyl,cycloalkyl(alkylene), cycloalkyl(heteroalkylene), heterocycloalkyl,heterocycloalkyl(alkylene), heterocycloalkyl(heteroalkylene),heteroaryl, heteroaryl(alkylene), and heteroaryl(heteroalkylene).

In addition, in any of the above-described aspects, unless otherwiseinconsistent therewith, the present invention provides compounds ofstructure (I) wherein R¹ is selected from halogen, heteroalkyl or amino,R² is H, R³ is aryl and R⁴ is H.

In addition, the present invention provides each of the followingcompounds, either separately (i.e., in isolation) or in any combination:6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide;N-(4-fluoro-phenyl)-6-(2-hydroxy-ethylamino)-1-oxy-nicotinamide;6-bromo-N-(4-fluoro-phenyl)-1-oxy-nicotinamide;5,6-dichloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide;6-ethanesulfonyl-N-(4-fluoro-phenyl)-1-oxy-nicotinamide;N-(4-fluorophenyl)-1-oxy-6-(propane-2-sulfonyl)-nicotinamide;N-(4-fluoro-phenyl)-6-methanesulfonyl-1-oxy-nicotinamide;6-benzenesulfonyl-N-(4-fluoro-phenyl)-1-oxy-nicotinamide;N-(4-fluoro-phenyl)-1-oxy-6-phenylmethanesulfonyl-nicotinamide;6-chloro-N-(3-chloro-4-fluoro-phenyl)-1-oxy-nicotinamide; and6-chloro-N-(4-iodo-phenyl)-1-oxy-nicotinamide.

In another aspect, the present invention provides composition comprisinga compound or compounds as set forth in any of the above-mentionedaspects, and a pharmaceutically acceptable carrier, adjuvant orincipient.

Furthermore, the present invention provides a method for antagonizingchemokine receptors comprising administering to a patient in needthereof an effective amount of a compound as set forth in any of theabove-mentioned aspects.

The present invention also provides a method for inhibiting achemokine-mediated cellular event comprising administering to a patientin need thereof an effective amount of a compound as set forth in any ofthe above-mentioned aspects. Optionally, the compounds inhibits a CXCR1receptor; and/or inhibits a CXCR2 receptor.

The present invention additionally provides a method for the treatmentof a disorder selected from Inflammatory Bowel Disease (IBD), psoriasis,rheumatoid arthritis, Acute Respiratory Distress Syndrome (ARDS),cancer, atherosclerosis, reperfusion injury, and graft vs. host disease.

In a further aspect, the present invention provides a method forinhibiting a G-protein-coupled, seven-transmembrane domain (7TM)receptor in a patient comprising administering to the patient a compoundas set forth in any of the above-mentioned aspects in an amounteffective to inhibit the receptor. Optionally, the compound modulatesthe binding of MIP-1β to a NPY cell receptor; and/or the compoundmodulates the binding of MIP-1β to a somatostatin cell receptor; and/orthe compound modulates the binding of MIP-1β to a CCR5 cell receptor.

The present invention also provides a method for treating aninflammation event, comprising administering to a patient in needthereof, through a therapeutically or prophylactically acceptablemanner, a therapeutically or pharmaceutically effective amount of acompound as set forth in any of the above-mentioned aspects. Optionally,the administration is selected from transdermal, oral, intravenous,intramuscular, vaginal, rectal, pulmonary, subcutaneous, sublingual andtransmucosal administration.

Furthermore, the present invention provides a method for identifying abinding partner to a compound as set forth in any of the above-mentionedaspects comprising: immoblizing proteins known to be involved in theTNF-α signaling pathway onto a suitable carrier; and passing a solutionof said compounds in isolation or mixture over said proteins andanalyzing for compound:protein complex formation using surface plasmonresonance (SPR).

In another aspect, the present invention provides a method foridentifying a binding partner to a compound as set forth in any of theabove-mentioned aspects comprising: providing said compound(s) bound toa solid support to provide solid phase compounds; contacting a cell orcell components with said solid phase compounds in isolation or mixture;removing uncomplexed cellular material, for example by gentle washingwith aqueous buffer; and recovering said binding partner from the solidphase compounds.

These and other related aspects of the present invention are set forthin more detail below.

DETAILED DESCRIPTION OF THE INVENTION

In one aspect, the present invention provides nicotinanilide-N-oxidecompounds having the structure (I):

and optical isomers, diastereomers, enantiomers, solvates andpharmaceutically acceptable salts thereof, wherein R¹ is selected fromR⁵ and R⁵-(C₁-C₆heteroalkylene)- where R⁵ is selected from hydrogen,halogen, alkyl, heteroalkyl, aryl, heteroaryl, carbocycle aliphatic ringand heterocycle aliphatic ring, amino or hydroxy; R² and R³ areindependently hydrogen, alkyl, heteroalkyl, aryl, aryl(akylene),heteroaryl, heteroaryl(alkylene), carbocycle, carbocycle(alkylene),heterocycle, and heterocycle(alkylene); each occurrence of R⁴ isindependently selected from halogen, alkyl, heteroalkyl, aryl,heteroaryl, carbocycle aliphatic ring and heterocycle aliphatic ring,amino or hydroxy; and n is 0, 1, 2 or 3. In addition, the presentinvention provides pharmaceutical compositions including saidnicotinanilide-N-oxide compounds, and method of using said compounds andcompositions, particularly in therapy.

Prior to setting forth a detailed description of the compounds,compositions and methods of the present invention, the followingdefinitions as used herein are provided.

Definition of Terms

“Alkyl” is a saturated or unsaturated, straight or branched, hydrocarbonchain. In various embodiments, the alkyl group has 1-18 carbon atoms,i.e., is a C1-C18 group, or is a C1-C12 group, a C1-C6 group, or a C1-C4group. Independently, in various embodiments, the alkyl group has zerobranches (i.e., is a straight chain), one branch, two branches, or morethan two branches. Independently, in one embodiment, the alkyl group issaturated. In another embodiment, the alkyl group is unsaturated. Invarious embodiments, the unsaturated alkyl may have one double bond, twodouble bonds, more than two double bonds, and/or one triple bond, twotriple bonds, or more than two triple bonds. Exemplary alkyl groupsinclude, without limitation, CH₃—, CH₃CH₂—, CH₂—CH—, CH₃CH₂CH₂—,CH₂(CH₃)CH₂—, CH₃C(CH₃)₂CH₂—. Alkyl chains may be substituted orunsubstituted. In one embodiment, the alkyl chains are unsubstituted. Inanother embodiment, the alkyl chain is substituted, e.g., with 1substituent (i.e., the alkyl group is monosubstituted), or 1-2substituents, or 1-3 substituents, or 1-4 substituents, etc. Whensubstituted with a heteroatom, the substituted alkyl group may bereferred to as a heteroalkyl.

“Alkylene” refers to a divalent alkyl radical, i.e., if hydrogen were tooccupy one open valence site of an alkylene group then a alkyl groupwould result. Exemplary alkylene groups include, without limitation,—CH₂—, —CH₂CH₂—, —CH═CH—, —CH₂CH₂CH₂—, —CH(CH₃)CH₂—, —CH₂C(CH₃)₂CH₂—. Aswith alkyl groups, the alkylene group may be substituted orunsubstituted. In one embodiment, the alkylene group is unsubstituted.In another embodiment, the alkylene group is substituted, e.g., with 1substituent (i.e., the alkylene group is monosubstituted), or 1-2substituents, or 1-3 substituents, or 1-4 substituents, etc. Whensubstituted with a heteroatom, the substituted alkylene group mayoptionally be referred to as a heteroalkylene. In one embodiment, thealkylene group is joined to an aryl group, so as to form anaryl(alkylene) group, also referred to as an aralkyl group. In oneembodiment, aryl(alkylene) refers to C₇-C₂₀ groups, such as benzyl,α-methylbenzyl, α,α-dimethylbenzyl, phenylethyl (a.k.a. phenethyl),phenylpropyl, phenylbutyl and phenylhexyl are exemplary aralkyl groups.In another embodiment, the aralkyl group is C₇-C₁₁. In otherembodiments, the alkylene group may be joined to a heteroaryl group (soas to form a heteroaryl(alkylene) group), a carbocycle group (so as toform a carbocycle(alkylene) group), and a heterocycle group (so as toform a heterocycle(alkylene) group).

“Aryl” is an aromatic hydrocarbon ring system. The ring system may bemonocyclic or polycyclic (i.e., bicyclic, tricyclic, etc.). In variousembodiments, the monocyclic aryl ring is C5-C10, or C5-C7, or C5-C6,where these carbon numbers refer to the number of carbon atoms that makeup the ring system. A C6 ring system, i.e., phenyl, is a preferred arylring. In various embodiments, the polycyclic ring is a bicyclic arylring, where preferred bicyclic aryl rings are C8-C12, or C9-C10. Anaphthyl ring, which has 10 carbon atoms, is a preferred polycyclic arylring. Aryl rings may be substituted or unsubstituted. In one embodiment,the aryl ring is unsubstituted. In another embodiment, the aryl ring issubstituted with 1 substituent (i.e., the aryl ring is monosubstituted),or 1-2 substituents, or 1-3 substituents, or 1-4 substituents, etc.

“Carbocyclic aliphatic ring,” also referred to as carbocycle orcycloalkyl, is a saturated or unsaturated, monocyclic or polycyclic(i.e., bicyclic, tricyclic, etc.) hydrocarbon ring. Carbocyclicaliphatic rings are not aromatic. A polycyclic hydrocarbon ring mayinclude fused, spiro or bridged ring structures. In various embodiments,the monocyclic carbocyclic aliphatic ring is a C3-C10, or a C4-C7, or aC5-C6 ring system. In various embodiments, the polycyclic carbocyclicaliphatic ring is a C6-C12, or a C9-C10 ring system. In one embodiment,the polycyclic ring is bicyclic. In another embodiment, the polycyclicring is bicyclic or tricyclic. Carbocyclic aliphatic rings includecyclopropylcyclopentyl, cyclohexyl, cyclohexenyl, cycloheptyl, andcyclooctyl. Carbocycles may be substituted or unsubstituted. In oneembodiment, the carbocycle is unsubstituted. In another embodiment, thecarbocycle is substituted with, e.g., 1 substituent (i.e., the alkylgroup is monosubstituted), or 1-2 substituents, or 1-3 substituents, or1-4 substituents, etc.

“Haloalkyl” is an alkyl chain substituted with one or more halogens. Apreferred haloalkyl is trifluoromethyl.

“Halogen” refers to fluoride, chloride, bromide or iodide. In apreferred embodiment, halogen refers to fluoride or chloride.

“Heteroalkyl” is a monovalent, saturated or unsaturated, straight orbranched, chain containing carbon and at least one heteroatom. Theheteroalkyl group may, in various embodiments, have one heteroatom, or1-2 heteroatoms, or 1-3 heteroatoms, or 1-4 heteroatoms. Heteroalkylchains may contain from 1 to 18 (i.e., 1-18) member atoms (carbon andheteroatoms) in the chain, and in various embodiments contain 1-12, or1-6, or 1-4 member atoms. Independently, in various embodiments, theheteroalkyl group has zero branches (i.e., is a straight chain), onebranch, two branches, or more than two branches. Independently, in oneembodiment, the heteroalkyl group is saturated. In another embodiment,the heteroalkyl group is unsaturated. In various embodiments, theunsaturated heteroalkyl may have one double bond, two double bonds, morethan two double bonds, and/or one triple bond, two triple bonds, or morethan two triple bonds. Heteroalkyl chains may be substituted orunsubstituted. In one embodiment, the heteroalkyl chain isunsubstituted. In another embodiment, the heteroalkyl chain issubstituted. A substituted heteroalkyl chain may have 1 substituent(i.e., be monosubstituted), or may have 1-2 substituents, or 1-3substituents, or 1-4 substituents, etc.

“Heteroalkylene” refers to an alkylene group wherein one or more of thecarbons is replaced with a heteroatom. Thus, the heteroalkylene group isa saturated or unsaturated, straight or branched chain divalent radicalthat contains at least one heteroatom. The heteroalkylene group may, invarious embodiments, have one heteroatom, or 1-2 heteroatoms, or 1-3heteroatoms, or 1-4 heteroatoms. Heteroalkylene chains may contain from1 to 18 (i.e., 1-18) member atoms (carbons and/or heteroatoms) in thechain, and in various embodiments contain 1-12, or 1-6, or 1-4 memberatoms. Independently, in various embodiments, the heteroalkylene grouphas zero branches (i.e., is a straight chain), one branch, two branches,or more than two branches. Independently, in one embodiment, theheteroalkylene group is saturated. In another embodiment, theheteroalkylene group is unsaturated. In various embodiments, theunsaturated heteroalkylene may have one double bond, two double bonds,more than two double bonds, and/or one triple bond, two triple bonds, ormore than two triple bonds.

“Heteroaryl” is an aromatic ring system containing carbon and at leastone heteroatom, that is, the heteroaryl group includes at least onearomatic ring containing a heteroatom, i.e., a heteroaryl ring. Theheteroaryl ring may, in various embodiments, have 1 heteroatom, 1-2heteroatoms, 1-3 heteroatoms, or 1-4 heteroatoms in the heteroaryl ring.Heteroaryl groups may be monocyclic or polycyclic (i.e., bicyclic,tricyclic, etc.), where the polycyclic ring may contained fused, spiroor bridged ring junctions. In one embodiment, the heteroaryl ismonocyclic, while in another embodiment the heteroaryl group is selectedfrom monocyclic and bicyclic rings. Monocyclic heteroaryl rings maycontain from about 5 to about 10 member atoms (carbon and heteroatoms),preferably from 5-7, and most preferably from 5-6 member atoms in thering. Bicyclic heteroaryl groups may contain from about 8-12 memberatoms, or 9-10 member atoms in the rings. In a polycyclic heteroarylgroup, at least one ring contains heteroatoms, and theheteroatom-containing ring is aromatic. The additional rings may or maynot, independently in each ring, contain heteroatom(s). If an additionalring contains heteroatom(s), then in various embodiments an additionalring has 1 heteroatom, 1-2 heteroatoms, or 1-3 heteroatoms.Independently, the additional rings may or may not be aromatic, that is,they may be saturated, unsaturated but not aromatic, or aromatic. Theheteroaryl group may be unsubstituted or substituted. In one embodiment,the heteroaryl group is unsubstituted. In another embodiment, theheteroaryl group is substituted. The substituted heteroaryl group may,in various embodiments, contain 1 substituent, 1-2 substituents, 1-3substituents, or 1-4 substituents. Exemplary heteroaryl groups include,without limitation, benzofuran, benzothiophene, furan, imidazole,indole, isothiazole, oxazole, pyrazine, pyrazole, pyridazine, pyridine,pyrimidine, pyrrole, quinoline, thiazole and thiophene.

“Heteroatom” is a nitrogen, sulfur, or oxygen atom. Groups containingmore than one heteroatom may contain different heteroatoms, i.e.,heteroatoms are selected on an independent basis upon each occurrence.

“Heterocyclic aliphatic ring,” also referred to as heterocyclyl orcycloheteroalkyl or heterocycloalkyl, is a saturated or unsaturated,monocyclic or polycyclic (i.e., bicyclic, tricyclic, etc.) ringcontaining carbon and at least one heteroatom. Heterocyclic aliphaticrings are not aromatic. The heterocyclic aliphatic ring may, in variousembodiments, have one heteroatom, or 1-2 heteroatoms, or 1-3heteroatoms, or 1-4 heteroatoms, etc. In one embodiment, theheterocyclic aliphatic ring is monocyclic, where the monocyclic ring mayhave 3-10, or 4-7, or 5-6 member atoms. In another embodiment, theheterocyclic aliphatic ring is polycyclic, where in various embodiments,the ring may be bicyclic, or may be tricyclic, or may be either bicyclicor tricyclic. A polycyclic ring system may have one or more fused, spiroor bridged ring systems. The polycyclic heterocyclic aliphatic ringsystem may have 6-12, or 9-10 member atoms. The heterocyclic ring may beunsubstituted or substituted. In one embodiment, the heterocyclic ringis unsubstituted. In another embodiment, the heterocyclic ring issubstituted. The substituted heterocyclic ring may contain 1substituent, or 1-2 substituents, or 1-3 substituents, or 1-4substituents, etc. Exemplary heterocyclic aliphatic rings includepiperazyl, morpholinyl, tetrahydrofuranyl, tetrahydropyranyl andpiperidyl.

“Lower alkyl” is an alkyl chain comprised of 1-6, preferably 1-4 carbonatoms.

“Pharmaceutically acceptable salt” and “salts thereof” means organic orinorganic salts of the pharmaceutically important molecule. Apharmaceutically acceptable salt may involve the inclusion of anothermolecule such as an acetate ion, a succinate ion or other counterion.The counterion may be any organic or inorganic moiety that stabilizesthe charge on the parent compound. Furthermore, a pharmaceuticallyimportant organic molecule may have more than one charged atom in itsstructure. Situations where multiple charged atoms are part of themolecule may have multiple counterions. Hence, the molecule of apharmaceutically acceptable salt may contain one or more than onecharged atoms and may also contain, one or more than one counterion. Thedesired charge distribution is determined according to methods of drugadministration. Examples of pharmaceutically acceptable salts are wellknown in the art but, without limiting the scope of the presentinvention, exemplary presentations can be found in the Physician's DeskReference, The Merck Index, The Pharmacopoeia and Goodman & Gilman's ThePharmacological Basis of Therapeutics.

“Substituents” replace a hydrogen atom with a non-hydrogen atom on analkyl, heteroalkyl, aryl, heteroaryl, carbocycle, and/or heterocyclylgroup as defined herein. Where the substituent contains a heteroatom,that heteroatom may be at any acceptable oxidation state for thatparticular atom, e.g., sulfur as part of a substituent may vary from anoxidation state of −2 to +8, and may be part of a complex or chelate asin a sulfoxide a mercapto-phosphine or metal chelated in a thia-crownether. Suitable substituents that may be located on one or more of thesegroups include the following: alkoxy (i.e., alkyl-O—, e.g., methoxy,ethoxy, propoxy, butoxy, pentoxy), aryloxy (e.g., phenoxy,chlorophenoxy, tolyloxy, methoxyphenoxy, benzyloxy,alkyloxycarbonylphenoxy, alkyloxycarbonyloxy, acyloxyphenoxy), acyloxy(e.g., propionyloxy, benzoyloxy, acetoxy), carbamoyloxy, carboxy,mercapto, alkylthio, acylthio, arylthio (e.g., phenylthio,chlorophenylthio, alkylphenylthio, alkoxyphenylthio, benzylthio,alkyloxycarbonyl-phenylthio), amino (e.g., amino, mono- and di-C1-C3alkanylamino, methylphenylamino, methylbenzylamino, C1-C3 alkanylamido,acylamino, carbamamido, ureido, guanidino, nitro and cyano). Moreover,any substituent may have from 1-5 further substituents attached thereto.

“Amino” means a trivalent amine substituted with up to 2 alkyl groups asdefined above or with 1 alkyl group and a hydrogen group, or with onearyl and one alkyl groups, or with two aryl groups, or with two or morehydrogen groups or with the substitution required to complete thenitrogen's valence requirements. “Amino” further includes amino saltswhere the nitrogen is hypervalent, having four bonds and may or may nothave a charge and a counterion. The counterion, when present, may be anexternal inorganic and/or organic counterion and/or may be an internalcounterion. Inorganic counterions include, for example, anions such ashalo anions and other non-metal anions. Examples of organic counterionsinclude, for example, anionic organic moieties such as acetate, citrateand other anionic organic moieties.

As stated previously, the present invention providesnicotinanilide-N-oxide compounds having the structure (I):

The inventive compounds include optical isomers, diastereomers,enantiomers, solvates and pharmaceutically acceptable salts includingthe structure (I). The structure (I) does not show an optically activecenter. Accordingly, the optical isomers of the present invention havean optically active center in one or more of R¹, R², R³ and R⁴. When anoptically active center is present in an inventive compound, theinvention provides for mixtures of optically active compounds as well asisolated optically active compounds. An optically active compound may beisolated by, for example, chiral resolution techniques. Likewise, thepresence of diastereomeric and enantiomeric forms of the compounds ofstructure (I) depend on the identities of R¹, R², R³ and R⁴. Again, whenthe compounds of the invention may be present in diastereomerically pureor mixed form, or enantiomerically pure or mixed form, the presentinvention provides for both mixtures and isolated compounds.

Pharmaceutically acceptable salts of the compounds of structure (I) maybe formed, depending on whether one or more of R¹, R², R³ and R⁴ containan acidic or basic site. Suitable counterions for the pharmaceuticallyacceptable salts include chloride, sulfate, phosphate, citrate,fumarate, methanesulfonate, acetate, tartrate, maleate, lactate,mandelate, salicylate, succinate and other salts known in the art.

The value of n in the compounds of the invention may be 0, 1, 2 or 3. Inone aspect of the invention n is 0, such that the anilide ring containsthree hydrogen substituents in addition to R¹. In another aspect, n is 1such that the anilide rings contains 2 hydrogen substituents. In anotheraspect, n is 2 such that the anilide rings contains 1 hydrogensubstituents. In another aspect, n is selected from 0 and 1. In anotheraspect, n is selected from 0, 1 and 2. In another aspect, n is selectedfrom 1 and 2.

The identity of R¹ is selected from R⁵ and R⁵-(C₁-C₆heteroalkylene)-where R⁵ is selected from hydrogen, halogen, alkyl, heteroalkyl, aryl,heteroaryl, carbocycle aliphatic ring, heterocycle aliphatic ring, aminoand hydroxy. In one aspect, R¹ is R⁵ and does not includeR⁵-(C₁-C₆heteroalkylene)-. In another aspect, R¹ isR⁵-(C₁-C₆heteroalkylene)- and does not include R⁵ alone.

In another aspect, R¹ is R⁵—SO₂— (i.e., R¹ is R⁵-(C₁-C₆heteroalkylene)-where (C₁-C₆heteroalkylene) is SO₂) and R⁵ is selected from alkyl,heteroalkyl, aryl, carbocycle, aryl(alkylene), and carbocycle(alkylene).In various aspects, alkyl refers to C₁-C₁₀alkyl; heteroalkyl refers toC₁-C₁₀alkyl with 1, 2 or 3 heteroatoms selected from N, O and S; arylrefers to phenyl, substituted phenyl, naphthyl or substituted naphthyl;carbocycle refers to C₃-C₈carbocycle; and alkylene refers toC₁-C₁₀alkylene. In various other aspects, R¹ is selected from(C₁-C₆alkyl)SO₂—, PhSO₂—, fluorinatedphenylSO₂—, PhCH₂SO₂—,cyclopentylSO₂—, m-carboxyphenylSO₂—, m-methylphenylSO₂—, andHOOC—(C₁-C₄alkylene)SO₂—.

In another aspect, R¹ is selected from halogen, amino, hydrocarbylamino,dihydrocarbylamino, hydrocarbyloxy, hydrocarbylthio, heterocyclyl,(heteroalkyl)amino, and (heteroaryl)amino. In various aspects, R¹ isselected from amino, (C₁-C₆alkyl)(C₁-C₆alkyl)amino, PhNH—, PhCH₂NH—,

and HOCH₂CH₂NH—. In yet other aspects R¹ is selected from halide and(C₁-C₆alkyl)S—, where R¹ may be, in one aspect, chloride.

The compounds of the invention have amide substitution on the anilidering, and in particular have a —C(═O)NR²R³ substituent on the anilidering. The R² and R³ groups are independently selected from hydrogen,alkyl, heteroalkyl, aryl, aryl(akylene), heteroaryl,heteroaryl(alkylene), carbocycle, carbocycle(alkylene), heterocycle, andheterocycle(alkylene). In one aspect, R² is hydrogen. In another aspect,R² is hydrogen when n is 0. In another aspect, R² is hydrogen when n is1.

In various aspects, and optionally in addition to the other particularaspects defined above, the compounds of the invention have R³ selectedfrom aryl, aryl(alkylene), heteroaryl, and heteroaryl(alkylene). In oneaspect, R³ is aryl. In another aspect, R³ is para-fluorophenyl. Inanother aspect, R² is hydrogen, n is zero, and R³ is para-fluorophenyl,so that the invention provides compounds having structure (II):

In another aspect, the present invention provides compounds of structure(I) wherein R³ is benzyl or phenyl, the benzyl or phenyl having 0, 1, 2,3 or 4 substituents selected from alkoxy, alkoxycarbonyl, alkyl,alkylamido, alkylcarbonyl, amido, benzyl optionally substituted withhalogen, benzyloxy, carboxy, cyano, dialkylamido, haloalkyl,haloalkyloxy, halogen, hydroxy, nitro, oxoalkyl, phenyl optionallysubstituted with halogen, thioalkyl, thiocyanate, and thiohaloalkyl,where in a preferred aspect R² is defined as hydrogen.

In another aspect, the present invention provides compound of structure(I) wherein R³ is selected from cycloalkyl, cycloalkyl(alkylene),cycloalkyl(heteroalkylene), heterocycloalkyl,heterocycloalkyl(alkylene), heterocycloalkyl(heteroalkylene),heteroaryl, heteroaryl(alkylene), and heteroaryl(heteroalkylene), wherein a preferred aspect R² is defined as hydrogen.

In compounds of the invention, R⁴ is independently selected fromhalogen, alkyl, heteroalkyl, aryl, heteroaryl, carbocycle aliphatic ringand heterocycle aliphatic ring, amino and hydroxy. In one aspect, R⁴ isindependently selected from halogen, alkyl, heteroalkyl, amino andhydroxy. In one aspect, R⁴ is selected from halogen and alkyl.

Nicotinanilide-N-oxides of the present invention may be prepared asdepicted in Scheme 1. The starting material 1 in Scheme 1 is anactivated nicotinic acid, where Y is a suitable carbonyl-activatinggroup such as halogen, 1-hydroxybenzotriazole,7-aza-1-hydroxybenzotriazole, or other leaving group capable of beingdisplaced by an amine. Also in 1, n is 0, 1, 2 or 3, and R¹ is either R¹or a synthetic precursor to R¹, where R¹ is selected from R⁵ andR⁵-(C₁-C₆heteroalkylene)- and R⁵ is selected from hydrogen, halogen,alkyl, heteroalkyl, aryl, heteroaryl, carbocycle aliphatic ring andheterocycle aliphatic ring, amino or hydroxy. In 1, each occurrence ofR⁴ is independently selected from halogen, alkyl, heteroalkyl, aryl,heteroaryl, carbocycle aliphatic ring and heterocycle aliphatic ring,amino or hydroxy; or is a suitable synthetic precursor to a listed R⁴group.

Compounds of formula 1 are either commercially available from standardchemical supply houses, or may be readily prepared from thecorresponding commercially available nicotinic acids upon treatment withan activating agent, e.g., thionyl chloride. Alternatively, they may beprepared by methodology known to one of skill in the art, based oninformation in the chemical and patent literature, and compounds knownin the literature. See, e.g., Arch. Pharm. (Weinheim Ger.) 290:20-25,1957; J. Fluorine Chemistry, 101(1):45-60, 2000; J. Chem. Soc.5045-5048, 1965; J. Chem. Soc. 73:234, 1898; J. Med. Chem.35(3):518-525, 1992; U.S. Pat. Nos. 3,950,160, 3,766,195 and 3,637,716;German Patent DE 25 38 950, and Great Britain Patent GB 1,134,651.

Commercially available chemicals may be obtained from standardcommercial sources including Acros Organics (Pittsburgh Pa.), AldrichChemical (Milwaukee Wis., including Sigma Chemical and Fluka), ApinChemicals Ltd. (Milton Park UK), Avocado Research (Lancashire U.K.), BDHInc. (Toronto, Canada), Bionet (Cornwall, U.K.), Chemservice Inc. (WestChester Pa.), Crescent Chemical Co. (Hauppauge N.Y.), Eastman OrganicChemicals, Eastman Kodak Company (Rochester N.Y.), Fisher Scientific Co.(Pittsburgh Pa.), Fisons Chemicals (Leicestershire UK), FrontierScientific (Logan Utah), ICN Biomedicals, Inc. (Costa Mesa Calif.), KeyOrganics (Cornwall U.K.), Lancaster Synthesis (Windham N.H.), MaybridgeChemical Co. Ltd. (Cornwall U.K.), Parish Chemical Co. (Orem Utah),Pfaltz & Bauer, Inc. (Waterbury CN), Polyorganix (Houston Tex.), PierceChemical Co. (Rockford Ill.), Riedel de Haen AG (Hannover, Germany),Spectrum Quality Product, Inc. (New Brunswick, N.J.), TCI America(Portland Oreg.), Trans World Chemicals, Inc. (Rockville Md.), and WakoChemicals USA, Inc. (Richmond Va.).

Compounds described in the chemical literature as referred to herein maybe identified though various reference books and databases. Suitablereference books and treatise that detail the synthesis of reactantsuseful in the preparation of compounds of the present invention, orprovide references to articles that describe the preparation, includefor example, Fuhrhop, J. and Penzlin G. “Organic Synthesis: Concepts,Methods, Starting Materials”, Second, Revised and Enlarged Edition(1994) John Wiley & Sons ISBN: 3-527-29074-5; Hoffman, R. V. “OrganicChemistry, An Intermediate Text” (1996) Oxford University Press, ISBN0-19-509618-5; Larock, R. C. “Comprehensive Organic Transformations: AGuide to Functional Group Preparations” 2nd Edition (1999) Wiley-VCH,ISBN: 0-471-19031-4; March, J. “Advanced Organic Chemistry: Reactions,Mechanisms, and Structure” 4th Edition (1992) John Wiley & Sons, ISBN:0-471-60180-2; Otera, J. (editor) “Modern Carbonyl Chemistry” (2000)Wiley-VCH, ISBN: 3-527-29871-1; Patai, S. “Patai's 1992 Guide to theChemistry of Functional Groups” (1992) Interscience ISBN: 0-471-93022-9;Quin, L. D. et al. “A Guide to Organophosphorus Chemistry” (2000)Wiley-Interscience, ISBN: 0-471-31824-8; Solomons, T. W. G. “OrganicChemistry” 7th Edition (2000) John Wiley & Sons, ISBN: 0-471-19095-0;Stowell, J. C., “Intermediate Organic Chemistry” 2nd Edition (1993)Wiley-Interscience, ISBN: 0-471-57456-2; “Industrial Organic Chemicals:Starting Materials and Intermediates: An Ullmann's Encyclopedia” (1999)John Wiley & Sons, ISBN: 3-527-29645-X, in 8 volumes; “OrganicReactions” (1942-2000) John Wiley & Sons, in over 55 volumes; and“Chemistry of Functional Groups” John Wiley & Sons, in 73 volumes.Specific and analogous reactants may also be identified through theindices of known chemicals prepared by the Chemical Abstract Service ofthe American Chemical Society, which are available in most public anduniversity libraries, as well as through on-line databases (the AmericanChemical Society, Washington, D.C., www.acs.org may be contacted formore details). Chemicals that are known but not commercially availablein catalogs may be prepared by custom chemical synthesis houses, wheremany of the standard chemical supply houses (e.g., those listed above)provide custom synthesis services.

As shown in Scheme 1, the nicotinic acid derivative 1 couples with aprimary or secondary amine (HNR²R³) to provide an amide intermediate 2.The coupling reaction is typically carried out in a suitable solvent,e.g., dichloromethane (DCM), in the presence of a suitable base, e.g. atertiary amine such as diisopropylethylamine (DIEA), at a suitablereaction temperature, e.g., room temperature (rt).

In amines of the formula HNR²R³, R² and R³ are independently selectedfrom hydrogen, alkyl, heteroalkyl, aryl, aryl(akylene), heteroaryl,heteroaryl(alkylene), carbocycle, carbocycle(alkylene), heterocycle, andheterocycle(alkylene). Many such amines are available from chemicalsupply houses, or may be prepared by methodology well known in thechemical literature.

The intermediate 2 may be oxidized under suitable conditions to give thenicotinanilide N-oxide 3. For example, 2 may be reacted with a suitableoxidizing reagent, e.g., aqueous hydrogen peroxide, in a suitablesolvent such as acetic acid or trifluoroacetic acid at a suitablereaction temperature, e.g., between about rt and about 70° C. Ifdesired, the nicotinanilide N-oxide 3 may be reacted under suitablereaction conditions to change any one or more of R¹, R², R³ and R⁴ andso form nicotinanilide N-oxide 3a, where R¹, R², R³ and R⁴ in 3a are asdefined above.

Alternatively, the intermediate 2 may be reacted under suitable reactionconditions to change any one or more of R¹, R², R³ and R⁴ and so providethe intermediate 2a. This intermediate 2a may then be exposed tooxidizing reaction conditions to form nicotinanilide N-oxide 3a. In eachof 2 and 2a, R¹, R², R³ and R⁴ are as defined above.

For example, when R¹ in intermediate 2 is a leaving group, e.g., halide,the intermediate 2 may be treated with a nucleophile that replaces thehalide with another R¹ group. Suitable conditions for this type oftransformation involve performing the reaction in a suitable solvent,e.g., tetrahydrofuran (THF), at a temperature, e.g., between roomtemperature and about 90° C., and optionally under pressure, e.g., asealed tube. Suitable nucleophiles include, but are not limited to,organoamines (e.g., dimethylamine, benzylamine, imidazole), alkoxides(e.g., sodium methoxide) and thiolates (e.g., sodium thiomethoxide).

The oxidizing conditions that convert 2 to 3, or 2a to 3a, maysimultaneously oxidize one or more of the R¹, R², R³ and R⁴ groups. Forinstance, when R¹ is —NR⁵R⁶ or —SR⁵, the resulting intermediate may beoxidized under the same conditions as above to give the 6-N-oxide or the6-sulfone, respectively.

Scheme 2 illustrates another route to nicotinanilide N-oxides 3.

As shown in Scheme 2, nicotinic acid 4 is oxidized under suitableconditions as described above (e.g., with hydrogen peroxide), followedby coupling to a primary or secondary amine, HNR²R³. Suitable conditionsfor this type of coupling involve performing the reaction in a suitablesolvent, e.g. THF, in the presence of a suitable coupling agent, e.g.2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline (EEDQ), and if necessary,a suitable tertiary amine such as DIEA. Nicotinic acids 4 arecommercially available or may be readily prepared from commerciallyavailable nicotinic acids.

C. Pharmaceutical Compositions

In another aspect, the present invention provides a compositioncontaining a nicotinanilide N-oxide compound of formula (I) in admixturewith a pharmaceutically acceptable adjuvant, carrier, diluent orexcipient, i.e., the present invention provides a pharmaceuticalcomposition containing a compound of formula (I). The pharmaceuticalcomposition may contain optional ingredient(s) if desired.

The pharmaceutical compositions of the present invention may be in anyform which allows for the composition to be administered to a patient.Typical routes of administration include, without limitation, oral,topical, parenteral, sublingual, rectal, vaginal, and intranasal. Theterm parenteral as used herein includes subcutaneous injections,intravenous, intramuscular, intrasternal injection or infusiontechniques. Pharmaceutical composition of the invention are formulatedso as to allow the active ingredients contained therein to bebioavailable upon administration of the composition to a patient.Compositions that will be administered to a patient take the form of oneor more dosage units, where for example, a tablet may be a single dosageunit, and a container of nicotinanilide N-oxide in aerosol form may holda plurality of dosage units.

The composition may be in the form of a solid, liquid or gas (aerosol).In one aspect, the carrier(s) are particulate, so that the compositionsare, for example, in tablet or powder form. The carrier(s) may beliquid, with the compositions being, for example, an oral syrup orinjectable liquid. In addition, the carrier(s) may be gaseous, so as toprovide an aerosol composition useful in, e.g., inhalatoryadministration.

When intended for oral administration, the composition is preferably ineither solid or liquid form, where semi-solid, semi-liquid, suspensionand gel forms are included within the forms considered herein as eithersolid or liquid.

As a solid composition for oral administration, the composition may beformulated into a powder, granule, compressed tablet, pill, capsule,chewing gum, wafer or the like form. Such a solid composition willtypically contain one or more inert diluents or edible carriers. Inaddition, one or more of the following adjuvants may be present: binderssuch as carboxymethylcellulose, ethyl cellulose, microcrystallinecellulose, gum tragacanth or gelatin; excipients such as starch, lactoseor dextrins, disintegrating agents such as alginic acid, sodiumalginate, Primogel, corn starch and the like; lubricants such asmagnesium stearate or Sterotex; glidants such as colloidal silicondioxide; sweetening agents such as sucrose or saccharin, a flavoringagent such as peppermint, methyl salicylate or orange flavoring, and acoloring agent.

When the composition is in the form of a capsule, e.g., a gelatincapsule, it may contain, in addition to materials of the above type, aliquid carrier such as polyethylene glycol or a fatty oil.

The composition may be in the form of a liquid, e.g., an elixir, syrup,solution, emulsion or suspension. The liquid may be for oraladministration or for delivery by injection, as two examples. Whenintended for oral administration, preferred composition contain, inaddition to the present compounds, one or more of a sweetening agent,preservatives, dye/colorant and flavor enhancer. In a compositionintended to be administered by injection, one or more of a surfactant,preservative, wetting agent, dispersing agent, suspending agent, buffer,stabilizer and isotonic agent may be included.

The liquid pharmaceutical compositions of the invention, whether they besolutions, suspensions or other like form, may include one or more ofthe following adjuvants: sterile diluents such as water for injection,saline solution, preferably physiological saline, Ringer's solution,isotonic sodium chloride, fixed oils such as synthetic mono ordigylcerides which may serve as the solvent or suspending medium,polyethylene glycols, glycerin, propylene glycol or other solvents;antibacterial agents such as benzyl alcohol or methyl paraben;antioxidants such as ascorbic acid or sodium bisulfite; chelating agentssuch as ethylenediaminetetraacetic acid; buffers such as acetates,citrates or phosphates and agents for the adjustment of tonicity such assodium chloride or dextrose. The parenteral preparation can be enclosedin ampoules, disposable syringes or multiple dose vials made of glass orplastic. Physiological saline is a preferred adjuvant. An injectablepharmaceutical composition is preferably sterile.

A liquid compositions intended for either parenteral or oraladministration should contain an amount of the inventive compound suchthat a suitable dosage will be obtained. Typically, this amount is atleast 0.01% of a compound of the invention in the composition. Whenintended for oral administration, this amount may be varied to bebetween 0.1 and about 70% of the weight of the composition. Preferredoral compositions contain between about 4% and about 50% of the activevanadium(V) complex. Preferred compositions and preparations accordingto the present invention are prepared so that a parenteral dosage unitcontains between 0.01 to 1% by weight of active compound.

The pharmaceutical composition may be intended for topicaladministration, in which case the carrier may suitably comprise asolution, emulsion, ointment or gel base. The base, for example, maycomprise one or more of the following: petrolatum, lanolin, polyethyleneglycols, bee wax, mineral oil, diluents such as water and alcohol, andemulsifiers and stabilizers. Thickening agents may be present in apharmaceutical composition for topical administration. If intended fortransdermal administration, the composition may include a transdermalpatch or iontophoresis device. Topical formulations may contain aconcentration of the inventive compound of from about 0.1 to about 10%w/v (weight per unit volume).

The composition may be intended for rectal administration, in the form,e.g., of a suppository which will melt in the rectum and release thedrug. The composition for rectal administration may contain anoleaginous base as a suitable nonirritating excipient. Such basesinclude, without limitation, lanolin, cocoa butter and polyethyleneglycol.

The composition may include various materials which modify the physicalform of a solid or liquid dosage unit. For example, the composition mayinclude materials that form a coating shell around the activeingredients. The materials which form the coating shell are typicallyinert, and may be selected from, for example, sugar, shellac, and otherenteric coating agents. Alternatively, the active ingredients may beencased in a gelatin capsule.

The composition in solid or liquid form may include an agent which bindsto the nicotinanilide N-oxide compounds of the invention and therebyassists in the delivery of the active compound. Suitable agents whichmay act in this capacity include a monoclonal or polyclonal antibody, aprotein or a liposome.

Materials used in preparing the pharmaceutical compositions should bepharmaceutically pure and non-toxic in the amounts used. It will beevident to those of ordinary skill in the art that the optimal dosage ofthe active ingredient(s) in the pharmaceutical composition will dependon a variety of factors. Relevant factors include, without limitation,the type of subject (e.g., human), the particular form of the activeingredient, the manner of administration and the composition employed.

The pharmaceutical composition of the present invention may consist ofgaseous dosage units, e.g., it may be in the form of an aerosol. Theterm aerosol is used to denote a variety of systems ranging from thoseof colloidal nature to systems consisting of pressurized packages.Delivery may be by a liquefied or compressed gas or by a suitable pumpsystem which dispenses the active ingredients. Aerosols of compounds ofthe invention may be delivered in single phase, bi-phasic, or tri-phasicsystems in order to deliver the active ingredient(s). Delivery of theaerosol includes the necessary container, activators, valves,subcontainers, and the like, which together may form a kit. Preferredaerosols may be determined by one skilled in the art, without undueexperimentation.

Whether in solid, liquid or gaseous form, the pharmaceutical compositionof the present invention may contain one or more known pharmacologicalagents used in the treatment of inflammation.

The pharmaceutical compositions may be prepared by methodology wellknown in the pharmaceutical art. For example, a composition intended tobe administered by injection can be prepared by combining anicotinanilide N-oxide compounds of formula (I) with water so as to forma solution. A surfactant may be added to facilitate the formation of ahomogeneous solution or suspension. Surfactants are compounds thatnon-covalently interact with the nicotinanilide N-oxide compound so asto facilitate dissolution or homogeneous suspension of the compound inthe aqueous delivery system.

D. Biological Applications

The present invention provides nicotinanilide N-oxide, compositionscontaining a nicotinanilide N-oxide, and methods of using nicotinanilideN-oxide compounds to inhibit chemokine-mediated cellular eventsinvolving IL-8, including IL-8a (CXCR1 receptor) and IL-8b (CXCR2receptor). Thus, in one aspect, the present invention provides a methodto modulate binding of IL-8 to cell receptors, and/or modulate theconsequential intracellular events comprising administering to a subjectin a need thereof an effective amount of a nicotinanilide N-oxidecompounds of formula (I). Thus, in one aspect, the present inventionprovides a method for the inhibition of IL-8 or other CXC chemokinesbinding to CXCR1 and/or CXCR2 receptors comprising administering aneffective amount of a compound of formula (I) to a subject in needthereof. In another aspect, the present invention provides a method forreducing the levels of IL-8 within a subject comprising administering toa subject in need thereof an effective amount of a compound of formula(I). In another aspect, the present invention provides a method fortreating, preventing, or treating and/or preventing one or more ofinflammatory and autoimmune diseases such as Inflammatory Bowel Disease(IBD), psoriasis, rheumatoid arthritis, Acute Respiratory DistressSyndrome (ARDS), cancer, atherosclerosis, reperfusion injury, and graftvs. host disease, comprising administering to a subject in need thereofan effective amount of a compound of formula (I).

In another aspect, the present invention provides a method of usingnicotinanilide N-oxide compounds, and compositions comprisingnicotinanilide N-oxide compounds, to inhibit G-protein-coupled,seven-transmembrane domain (7TM) receptors. Thus, in one aspect, thepresent invention provides a method to modulate the binding of MIP-1β toCCR5 cell receptors. In another aspect, the present invention provides amethod to modulate the binding of Peptide YY (PYY) to NPY cellreceptors. In another aspect, the present invention provides a method tomodulate the binding of somatostatin to somatostatin cell receptors. Inone aspect, the nicotinanilide N-oxide compound modulates by reducingthe effective binding of MIP-1 to cell receptor.

The present invention provides a method for treating an inflammationevent, comprising administering to a patient in need thereof, through atherapeutically or prophylactically acceptable manner, a therapeuticallyor pharmaceutically effective amount of the compound of formula (I).Administering may be selected from transdermal, oral, intravenous,intramuscular, vaginal, rectal, pulmonary, subcutaneous, sublingual andtransmucosal administration.

The “effective amount” or “therapeutically effective amount” of acompound of the present invention will depend on the route ofadministration, the type of mammal being treated, and the physicalcharacteristics of the specific mammal under consideration. Thesefactors and their relationship to determining this amount are well knownto skilled practitioners in the medical arts. This amount and the methodof administration can be tailored to achieve optimal efficacy but willdepend on such factors as weight, diet, concurrent medication and otherfactors which those skilled in the medical arts will recognize.

In another aspect, the present invention provides a method foridentifying a binding partner to a nicotinanilide-N-oxide compound asdisclosed herein, where the method comprises: immoblizing protein knownto be involved in the TNF-α signaling pathway onto a suitable carrier;and passing a solution of said compounds in isolation or mixture oversaid protein and analyzing for compound:protein complex formation usingsurface plasmon resonance (SPR). This method may be performed in analogyto the method described in Karlsson, R et al. “Biosensor Analysis ofDrug-Target Interactions: Direct and Competitive Binding Assays forInvestigation of Interactions Between Thrombin and Thrombin Inhibitors”Anal. Biochem. 2000, 278(1), 1-13. For other examples of identifyingsmall molecule-protein interactions using SPR see the Biacore website:http://www.biacore.com.

In another aspect, the present invention provides a method foridentifying a binding partner to a nicotinanilide N-oxide compound asdisclosed herein, where the method comprises: contacting a cell or cellcomponents with said nicotinanilide N-oxide compound in isolation ormixture; removing uncomplexed cellular material, for example by gentlewashing with aqueous buffer; and recovering said binding partner fromthe compounds. The nicotinanilide N-oxide compound(s) are preferablybound to a solid support. See, e.g., methodology reported in Shimizu, Net al. “High Performance Affinity Beads for Identifying Drug Receptors”Nature Biotechnology, 2000, 18(8), 877-881).

As to each publication or patent referenced herein, that publication orpatent is incorporated herein by reference in its entirety for allpurposes.

From the foregoing it will be appreciated that, although specificembodiments of the invention have been described herein for purposes ofillustration, various modifications may be made without deviating fromthe spirit and scope of the invention. Accordingly, the invention is notlimited except as by the appended claims.

The following examples are offered by way of illustration, and not byway of limitation.

EXAMPLES Example 1 SYNTHESIS OF6-DIMETHYLAMINO-N-(4-FLUORO-PHENYL)-1-OXY-NICOTINAMIDE

A. 6-Chloro-N-(4-fluoro-phenyl)-nicotinamide

To a suspension of 6-chloronicotinoyl chloride (10.5 g, 59.7 mmole) indry dichloromethane (100 mL) was added 4-fluoroaniline (5.6 mL, 59.7mmole), followed by the dropwise addition of N,N-diisopropylethylamine(21 mL, 119 mmol). After stirring for 90 min at room temperature, themixture was diluted with ethyl acetate (20 mL) and washed with water.The combined organic extracts were dried over anhydrous sodium sulfate,filtered, and concentrated in vacuo. Purification by recrystallizationusing ethyl acetate/hexanes gave 13.8 g (92%) of a white solid. ¹H NMR(300 MHz, CDCl₃) δ 7.09-7.12 (m, 2H), 7.47-7.50 (m, 1H), 7.56-60 (m,2H), 7.7 (bs, 1H), 8.15-8.18 (m, 1H), 8.84 (m, 1H); MS (EI) m/z 251.13(M+H)⁺.B. 6-Chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide

To a solution of 6-chloro-N-(4-fluoro-phenyl)-nicotinamide (2.06 g, 8.2mmole) in 30 mL of acetic acid was added 30 mL of aqueous hydrogenperoxide (30%). The mixture was warmed to 70° C. and stirred for 18hours, and then 20 mL of additional hydrogen peroxide (30%) was addedand stirred for 2 hours. The mixture was then cooled, concentrated invacuo and diluted with water (100 mL). Isolation of the precipitate byvacuum filtration afforded 0.43 g (20%) of the desired product as awhite solid: ¹H NMR (300 MHz, DMSO-d₆) δ 7.19 (m 2H), 7.75 (M, 3H), 7.95(M, 1H), 8.94 (d, 1H, J=2.2 Hz), 10.5 (s, 1H); MS (EI) m/z 267.19(M+H)⁺.C. 6-Dimethylamino-N-(4-fluoro-phenyl)-1-oxy-nicotinamide

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.016 g, 0.060 mmole)and dimethylamine (2 mL of a of 2.0 M solution in THF). The mixture washeated to 90° C. for 4 h, then cooled and poured into ethyl acetate andwater. The organic layer was removed, dried over sodium sulfate,filtered, and the solvents removed in vacuo. Purification by triturationusing ethyl acetate/hexanes gave 6 mg (36%) of the desired product as awhite solid: ¹H NMR (300 MHz, DMSO-d₆) 3.00 (m, 6H), 7.10 (m, 3H), 7.7(m, 3H), 8.68 (m, 1H), 10.26 (m, 1H); MS (EI) m/z 276.27 (M+H)⁺.

Example 2 SYNTHESIS OF6-BENZYLAMINO-N-(4-FLUORO-PHENYL)-1-OXY-NICOTINAMIDE

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.020 g, 0.070 mmole)and benzylamine (1 mL). The mixture was heated to 90° C. for 18 h, thencooled and poured into ether. The resulting precipitate was collected byvacuum filtration and purified by trituration with methanol/ethylacetate to give 16 mg (70%) of the desired product as a white solid. ¹HNMR (300 MHz, CD₃OD) δ 4.60 (m, 2H), 6.85 (m, 1H), 7.05 (m, 3H), 7.33(m, 4H), 7.63 (m, 3H), 7.87 (m, 1H), 8.73 (s, 1H) MS (EI) m/z 338.26(M+H)⁺.

Example 3 SYNTHESIS OFN-(4-FLUORO-PHENYL)-1-OXY-6-PHENYLAMINO-NICOTINAMIDE

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.020 g, 0.070 mmole)and aniline (1 mL). The mixture was heated to 90° C. for 18 h thencooled and poured into ether. The resulting precipitate was collected byvacuum filtration and purified by trituration with ethyl acetate to give13.1 mg (59%) of the desired product as a white solid. ¹H NMR (300 MHz,DMSO-d₆) δ 7.17 (m, 5H), 7.35 (m, 4H), 7.75 (m, 2H), 8.92 (d, 1H, J-2.2Hz), 9.75 (s, 1H), 10.24 (s, 1H); MS (EI) m/z 322.23 (M−H)⁻.

Example 4 SYNTHESIS OFN-(4-FLUORO-PHENYL)-6-METHYLAMINO-1-OXY-NICOTINAMIDE

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.020 g, 0.070 mmole)and methylamine (1 mL of a 2.0 M solution in water). The mixture washeated to 90° C. for 18 h, then cooled and poured into ether. Theresulting precipitate was collected by vacuum filtration and purified byflash chromatography (10% methanol in ammonia saturated dichloromethane)to give 5.6 mg (21%) of the desired product as a white solid. ¹H NMR(300 MHz, CD₃OD) δ 3.05 (s, 3H), 6.94 (m, 1H), 7.06 (m, 2H), 7.64 (m,2H), 8.00 (m, 1H), 8.70 (m, 1H); MS (EI) m/z 260.26 (M−H)⁻.

Example 5 SYNTHESIS OFN-(4-FLUORO-PHENYL)-6-IMIDAZOL-1-YL-1-OXY-NICOTINAMIDE

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.020 g, 0.070 mmole),imidazole (95 mg, 1.4 mmol), and THF (1 mL). The mixture was heated to90° C. for 18 h, then cooled and poured into cold water. The resultingprecipitate was collected by vacuum filtration to give 10.4 mg (46%) ofthe desired product as a white solid. ¹H NMR (300 MHz, DMSO-d₆) δ7.18,(m, 3H), 7.78 (m, 5H), 8.53 (bs, 1H), 8.96 (s, 1H), 10.56 (s, 1H); MS(EI) m/z 299.23 (M+H)⁺.

Example 6 SYNTHESIS OFN-(4-FLUORO-PHENYL)-6-(2-HYDROXY-ETHYLAMINO)-1-OXY-NICOTINAMIDE

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.020 g, 0.070 mmole),and ethanolamine (1 mL). The mixture was heated to 90° C. for 18 h, thencooled and poured into cold water. The resulting precipitate wascollected by vacuum filtration to give 4.3 mg (21%) of the desiredproduct as a white solid. MS (EI) m/z 292.26 (M+H)⁺.

Example 7 SYNTHESIS OFN-(4-FLUORO-PHENYL)-1-OXY-6-PYRROLIDIN-1-YL-NICOTINAMIDE

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.020 g, 0.070 mmole),pyrrolidine (0.031 mL, 0.375 mmol), and THF (1 mL). The mixture washeated to 90° C. for 18 h, then cooled and poured into ethyl acetate.The resulting precipitate was collected by vacuum filtration to give16.1 mg (70%) of the desired product as a white solid. MS (EI) m/z302.25 (M+H)⁺.

Example 8 SYNTHESIS OFN-(4-FLUORO-PHENYL)-1-OXY-6-PENTYLAMINO-NICOTINAMIDE

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.020 g, 0.070 mmole),amylamine (0.043 mL, 0.375 mmol), and THF (1 mL). The mixture was heatedto 90° C. for 18 h and then cooled and poured into water. The resultingprecipitate was collected by vacuum filtration and purified bytrituration with ethyl acetate/methanol to give 16.2 mg (68%) of thedesired product as a white solid. MS (EI) m/z 318.28 (M+H)⁺.

Example 9 SYNTHESIS OF 6-AMINO-N-(4-FLUORO-PHENYL)-1-OXY-NICOTINAMIDE

In a sealed tube was placed6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide (0.020 g, 0.070 mmole)and ammonium hydroxide (2 mL of a 14.8 M solution in water). The mixturewas heated to 90° C. for 18 h and then cooled and the volatiles removedin vacuo. The resulting precipitate was purified by flash chromatography(10% methanol in ammonia saturated dichloromethane to give 9.5 mg (51%)of the desired product as a white solid. MS (EI) m/z 248.25 (M+H)⁺.

Example 10 6-BROMO-N-(4-FLUORO-PHENYL)-1-OXY-NICOTINAMIDE

To 8 mL of trifluoracetic acid at 0° C. was added 1 mL of aqueoushydrogen peroxide (30%) over a 5 minute period, followed by the additionof 6-bromo-N-(4-fluoro-phenyl)-nicotinamide (0.1 g, 0.34 mmol) in oneportion. The mixture was warmed to 45° C. and stirred for 18 hours. Themixture was cooled diluted with water (1 mL) and concentrated in vacuo.Purification by trituration using hot ethyl acetate produced the desiredproduct as a white solid: MS (EI) m/z 312.89 (M+H)⁺.

Example 11 N-(4-FLUORO-PHENYL)-6-METHYL-1-OXY-NICOTINAMIDE

The title compound was prepared in a similar fashion to2-Chloro-N-(4-fluoro-phenyl)-6-methyl-1-oxy-nicotinamide in Example 14.MS (EI) m/z 247.1 (M+H)⁺.

Example 12 SYNTHESIS OF 6-CHLORO-N-SUBSTITUTED-1-OXY-NICOTINAMIDES

A. 6-Chloro-1-oxy-nicotinic acid

To 400 mL of trifluoracetic acid at 0° C. was added 50 mL of aqueoushydrogen peroxide (30%) over a 5 minute period, followed by the additionof 6-chloro-nicotinic acid (3.07 g, 19.5 mmole) in one portion. Themixture was warmed to 45° C. and stirred for 18 hours, and then 30 mL ofadditional aqueous hydrogen peroxide (30%) was added in one portion andstirred for 2 h. The mixture was cooled and concentrated in vacuo.Purification by trituration using hot ethyl acetate produced 2.18 g(64%) of the desired product as a white solid: ¹H NMR (300 MHz, DMSO-d₆)δ 8.65 (d, 1H, J=1.9 Hz), 7.85 (m, 1H), 7.7 (m, 1H), 3.3 (bs, 1H); MS(EI) m/z 174.13/176.13 (M+H)⁺.General Procedure a for the Synthesis of6-chloro-N-substituted-1-oxy-nicotinamides

To a suspension of 6-chloro-1-oxy-nicotinic acid (0.040 g, 0.23 mmol) indry chloroform (5 mL) was added a selected amine (0.3 mmol) followed bythe addition of (2-ethyoxy-1-ethoxycarbonyl-1,2-dihydroquinoline, 0.32mmole). After stirring for 24 hours at room temperature the mixture wasdiluted with hexanes and the solids isolated by vacuum filtration.Purification of the solids by trituration with ethyl acetate and hexanesafforded the desired 1-oxy-nicotinamides.

Compounds prepared by the general procedure outlined in Example 12 wheresynthesized from the appropriate amine, as set forth in Table 1. TABLE 1N-Benzhydryl-6-chloro-1-oxy-nicotinamide: MS (EI) m/z 339.21 (M + H)⁺.6-Chloro-N-(2-fluoro-phenyl)-1-oxy-nicotinamide: MS (EI) m/z 267.23 (M +H)⁺. 6-Chloro-N-(3-chloro-4-fluoro-phenyl)-1-oxy-nicotinamide: MS (EI)m/z 299.20 (M − H)⁻. 6-Chloro-N-(4-nitro-phenyl)-1-oxy-nicotinamide: MS(EI) m/z 292.24 (M − H)⁻.N-(3-Bromo-phenyl)-6-chloro-1-oxy-nicotinamide: MS (EI) m/z 325.16 (M −H)⁻. 6-Chloro-N-(4-fluoro-3-trifluoromethyl-phenyl)-1-oxy-nicotinamide:MS (EI) m/z 333.21 (M − H)⁻.6-Chloro-N-(3-fluoro-4-methyl-phenyl)-1-oxy-nicotinamide: MS (EI) m/z279.27 (M − H)⁻. 6-Chloro-N-(3-iodo-phenyl)-1-oxy-nicotinamide: MS (EI)m/z 373.12 (M − H)⁻.3-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-4-methoxy-benzoic acid:MS (EI) m/z 323.06 (M + H)⁺.6-Chloro-N-(3,4-dimethyl-phenyl)-1-oxy-nicotinamide: MS (EI) m/z 275.25(M − H)⁻. 6-Chloro-N-(3,4-difluoro-phenyl)-1-oxy-nicotinamide: MS (EI)m/z 283.22 (M − H)⁻. N-(4-Benzyloxy-phenyl)-6-chloro-1-oxy-nicotinamide:MS (EI) m/z 353.23 (M − H)⁻.6-Chloro-N-(3-chloro-4-iodo-phenyl)-1-oxy-nicotinamide: MS (EI) m/z407.06 (M − H)⁻.N-(4-Bromo-3-chloro-phenyl)-6-chloro-1-oxy-nicotinamide: MS (EI) m/z361.09 (M − H)⁻.N-(4-Bromo-3-methyl-phenyl)-6-chloro-1-oxy-nicotinamide: MS (EI) m/z341.15 (M − H)⁻. 6-Chloro-N-(3-methoxy-phenyl)-1-oxy-nicotinamide: MS(EI) m/z 277.26 (M − H)⁻.N-(4-Bromo-phenyl)-6-chloro-1-oxy-nicotinamide: MS (EI) m/z 327.11 (M −H)⁻. N-(3,5-Bis-trifluoromethyl-phenyl)-6-chloro-1-oxy-nicotinamide: MS(EI) m/z 383.15 (M − H)⁻.6-Chloro-1-oxy-N-(3-trifluoromethoxy-phenyl)-nicotinamide: MS (EI) m/z331.19 (M − H)⁻.6-Chloro-N-(4-fluoro-3-methyl-phenyl)-1-oxy-nicotinamide: MS (EI) m/z279.24 (M − H)⁻.6-Chloro-N-(3-fluoro-4-methoxy-phenyl)-1-oxy-nicotinamide: MS (EI) m/z295.22 (M − H)⁻. 6-Chloro-N-(4-methoxy-phenyl)-1-oxy-nicotinamide: MS(EI) m/z 277.23 (M − H)⁻.6-Chloro-1-oxy-N-(4-trifluoromethyl-phenyl)-nicotinamide: MS (EI) m/z315.19 (M − H)⁻. 6-Chloro-N-(2-hydroxy-phenyl)-1-oxy-nicotinamide: MS(EI) m/z 263.23 (M − H)⁻.6-Chloro-N-(4-fluoro-2-methyl-phenyl)-1-oxy-nicotinamide: MS (EI) m/z279.24 (M − H)⁻. 6-Chloro-N-(3,4-dichloro-phenyl)-1-oxy-nicotinamide: MS(EI) m/z 315.00 (M − H)⁻.6-Chloro-N-(3-fluoro-phenyl)-1-oxy-nicotinamide: MS (EI) m/z 265.11 (M −H)⁻. 6-Chloro-N-(4-iodo-phenyl)-1-oxy-nicotinamide: MS (EI) m/z 372.93(M − H)⁻. N-(4-tert-Butyl-phenyl)-6-chloro-1-oxy-nicotinamide: MS (EI)m/z 303.29 (M − H)⁻.6-Chloro-N-(4-chloro-3-trifluoromethyl-phenyl)-1-oxy-nicotinamide: MS(EI) m/z 349.15 (M − H)⁻.6-Chloro-N-(3-chloro-phenyl)-1-oxy-nicotinamide: MS (EI) m/z 281.19 (M −H)⁻. 6-Chloro-1-oxy-N-(3-trifluoromethyl-phenyl)-nicotinamide: MS (EI)m/z 315.20 (M − H)⁻. 6-Chloro-N-(3-ethoxy-phenyl)-1-oxy-nicotinamide: MS(EI) m/z 291.25 (M − H)⁻.N-(3-Carbamoyl-phenyl)-6-chloro-1-oxy-nicotinamide: MS (EI) m/z 290.24(M − H)⁻. N-(4-Acetyl-phenyl)-6-chloro-1-oxy-nicotinamide: MS (EI) m/z289.24 (M − H)⁻. N-benzyl-6-chloro-1-oxy-nicotinamide: MS (EI) m/z261.25 (M − H)⁻. 6-Chloro-N-(4-fluoro-benzyl)-1-oxy-nicotinamide: MS(EI) m/z 279.24 (M − H)⁻. 6-Chloro-N-cyclopentyl-1-oxy-nicotinamide: MS(EI) m/z 241.21 (M + H)⁺. 6-Chloro-N-cyclohexyl-1-oxy-nicotinamide: MS(EI) m/z 255.27 (M + H)⁺.6-Chloro-N-furan-2-ylmethyl-1-oxy-nicotinamide: MS (EI) m/z 251.26 (M −H)⁻. 6-Chloro-1-oxy-N-phenyl-nicotinamide: MS (EI) m/z 247.26 (M − H)⁻.3-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-benzoic acid tert-butylester: MS (EI) m/z 347.27 (M − H)⁻.3-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-benzoic acid: MS (EI) m/z291.19 (M − H)⁻.3-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-4-methyl-benzoic acid: MS(EI) m/z 307.09 (M + H)⁺.3-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-4-methoxy-benzoic acid:MS (EI) m/z 323.06 (M + H)⁺.5-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-isophthalic acid: MS (EI)m/z 337.05 (M + H)⁺.3-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-2,5,6-trifluoro-benzoicacid: MS (EI) m/z 346.97(M + H)⁺.6-Chloro-1-oxy-N-(3-trifluoromethylsulfanyl-phenyl)-nicotinamide: MS(EI) m/z 347.13 (M − H)⁻.6-Chloro-N-(3-methylsulfanyl-phenyl)-1-oxy-nicotinamide: MS (EI) m/z293.19 (M − H)⁻. N-(4-butyl-phenyl)-6-chloro-1-oxy-nicotinamide: MS (EI)m/z 303.17 (M − H)⁻.N-(3-bromo-4-trifluoromethoxy-phenyl)-6-chloro-1-oxy-nicotinamide: MS(EI) m/z 410.95 (M − H)⁻.6-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-2,3-difluoro-benzoicacid: MS (EI) m/z 327.07 (M − H)⁻.6-Chloro-N-(2,3-difluoro-phenyl)-1-oxy-nicotinamide: MS (EI) m/z 283.12(M − H)⁻. 6-Chloro-N-(4-chloro-phenyl)-1-oxy-nicotinamide: MS (EI) m/z281.07 (M − H)⁻. 6-Chloro-N-(3-cyano-phenyl)-1-oxy-nicotinamide: MS (EI)m/z 272.08 (M − H)⁻.6-Chloro-N-(3,4-dimethoxy-phenyl)-1-oxy-nicotinamide: MS (EI) m/z 307.09(M − H)⁻. 6-Chloro-1-oxy-N-(3,4,5-trimethoxy-phenyl)-nicotinamide: MS(EI) m/z 337.07 (M − H)⁻.6-Chloro-1-oxy-N-(4-thiocyanato-phenyl)-nicotinamide: MS (EI) m/z 304.05(M − H)⁻. 4-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-benzoic acidmethyl ester: MS (EI) m/z 305.05 (M − H)⁻.6-Chloro-N-(3,3-dimethyl-butyl)-1-oxy-nicotinamide: MS (EI) m/z 255.18(M − H)⁻. 6-Chloro-N-(2,4-difluoro-benzyl)-1-oxy-nicotinamide: MS (EI)m/z 297.12 (M − H)⁻.6-Chloro-1-oxy-N-(4-trifluoromethoxy-phenyl)-nicotinamide: MS (EI) m/z331.18 (M − H)⁻.5-[(6-Chloro-1-oxy-pyridine-3-carbonyl)-amino]-2-hydroxy-benzoic acid:m/z 307.07 (M − H)⁻.6-Chloro-N-(3,4-difluoro-benzyl)-1-oxy-nicotinamide: m/z 297.13 (M −H)⁻. 6-Chloro-N-(2-methylsulfanyl-phenyl)-1-oxy-nicotinamide: m/z 295.07(M + H)⁺. 6-Chloro-N-(4-methylsulfanyl-phenyl)-1-oxy-nicotinamide: m/z295.04 (M + H)⁺.

Example 13 SYNTHESIS OF2,6-DICHLORO-N-(4-FLUORO-PHENYL)-1-OXY-NICOTINAMIDE

To 16 mL of trifluoracetic acid at 0° C. was added 2 mL of aqueoushydrogen peroxide (30%) over a 5 minute period followed by the additionof 2,6-dichloro-N-(4-fluoro-phenyl)-nicotinamide (0.107 g, 0.377 mmole)in one portion. The mixture was warmed to room temperature and stirredfor 18 hours. The mixture was concentrated in vacuo. Purification bytrituration using hot ethyl acetate produced 8 mg (7%) of the desiredproduct as a white solid: MS (EI) m/z 299.16 (M−H)⁻.

Example 14 SYNTHESIS OF2-CHLORO-N-(4-FLUORO-PHENYL)-6-METHYL-1-OXY-NICOTINAMIDE

To 8 mL of trifluoracetic acid at 0° C. was added 1 mL of aqueoushydrogen peroxide (30%) over a 5 minute period followed by the additionof 2-chloro-N-(4-fluoro-phenyl)-6-methyl-nicotinamide (0.100 g, 0.379mmole) in one portion. The mixture was warmed to 50° C. and stirred for18 hours, and then 1 mL of additional aqueous hydrogen peroxide (30%)was added in one portion and stirred for 24 h. The mixture was cooledand concentrated in vacuo. Purification by reverse phase HPLC produced 4mg (4%) of the desired product as a white solid: MS (EI) m/z 279.15(M−H)⁻.

Example 15 SYNTHESIS OF5,6-DICHLORO-N-(4-FLUORO-PHENYL)-1-OXY-NICOTINAMIDE

To 8 mL of trifluoracetic acid at 0° C. was added 1 mL of aqueoushydrogen peroxide (30%) over a 5 minute period followed by the additionof 5,6-dichloro-N-(4-fluoro-phenyl)-nicotinamide (0.100 g, 0.35 mmole)in one portion. The mixture was warmed to 50° C. and stirred for 18hours, and then 1 mL of additional aqueous hydrogen peroxide (30%) wasadded in one portion and stirred for 24 h. The mixture was cooled andconcentrated in vacuo. Purification by trituration using methanol/ethylacetate produced 11 mg (10%) of the desired product as a white solid:299.14 (M−H)⁻.

Example 16 SYNTHESIS OF N-(4-FLUORO-PHENYL)-6-METHOXY-1-OXY-NICOTINAMIDE

To a suspension of 6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide(0.040 g, 0.15 mmole) in dry tetrahydrofuran (1 mL) was added sodiummethoxide (0.024 g, 0.045 mmol) and dry methanol (0.5 mL). Afterstirring for 1 hour at room temperature additional sodium methoxide(0.024 g, 0.045 mmol) was added and stirred for 15 minutes. The mixturewas diluted with ethyl acetate (20 mL) and washed with water. Thecombined organic extracts were dried over anhydrous sodium sulfate,filtered, and concentrated in vacuo. Purification by trituration usingethyl acetate gave 18 mg (46%) of the desired product as a white solid.MS (EI) m/z 263.21 (M+H)⁺.

Example 17 SYNTHESIS OFN-(4-FLUORO-PHENYL)-6-METHYLSULFANYL-1-OXY-NICOTINAMIDE

To a suspension of 6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide(0.033 g, 0.12 mmole) in dry tetrahydrofuran (1 mL) was added sodiumthiomethoxide (0.013 g, 0.18 mmol). After stirring for 1 h at roomtemperature the mixture was diluted with ice water and the solidsisolated by vacuum filtration to provide 26 mg (79%) of the desiredproduct as a white solid. MS (EI) m/z 277.17 (M−H)⁻.

Example 18 SYNTHESIS OF6-ETHYLSULFANYL-N-(4-FLUORO-PHENYL)-1-OXY-NICOTINAMIDE

To a suspension of 6-chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide(0.102 g, 0.38 mmole) in dry tetrahydrofuran (4 mL) was added sodiumthioethoxide (0.080 g, 0.96 mmol). The mixture was stirred for 3 hours.Approximately half of the volatiles were removed in vacuo and theremaining solids diluted with ice water and the solids isolated byvacuum filtration. The solids were purified by trituration using ethylacetate/methanol to provide 37 mg (37%) of the desired product as awhite solid. MS (EI) m/z 291.14 (M−H)⁻.

Example 19 SYNTHESIS OF 6-DIMETHYLAMINO-N-(4-FLUORO-PHENYL)-NICOTINAMIDEN-OXIDE

To a solution of 6-dimethylamino-N-(4-fluoro-phenyl)-nicotinamide (0.027g, 0.11 mmol, prepared as in Example 1) in 2 mL of drydichloromethane/methanol (4:1) was added 3-chloroperoxybenzoic acid(0.020 g, 0.12 mmol) at 0° C. The reaction mixture was then heated toreflux and additional 3-chloroperoxybenzoic acid (0.040 g, 0.24 mmol)was added. After heating for a total of 7 h the reaction mixture wascooled, diluted with chloroform and washed with aqueous sodium carbonate(1M), dried over sodium sulfate, and concentrated in vacuo. Purificationby flash chromatography, using 10% methanol/dichloromethane saturatedwith ammonia, yielded 4 mg (13%) of the desired product as a whitesolid. MS (EI) m/z 274.18 (M−H)⁻.

Example 20 SYNTHESIS OF6-ETHANESULFONYL-N-(4-FLUORO-PHENYL)-1-OXY-NICOTINAMIDE

A. 6-Ethylsulfanyl-N-(4-fluoro-phenyl)-nicotinamide

Into a round-bottomed flask equipped with a condenser was placed6-chloro-N-(4-fluoro-phenyl)-nicotinamide (0.20 g, 0.80 mmol),tetrahydrofuran (6 mL), and sodium thioethoxide (0.10 g, 1.2 mmol). Themixture was heated to reflux for 4 h at which time additional sodiumthioethoxide (0.10 g, 1.2 mmol) was added and stirred for 2 additionalhours. The reaction mixture was then cooled and poured into ice waterand the solids isolated by vacuum filtration to provide 0.15 g (68%) ofthe product as a white solid. MS (EI) m/z 275.2 (M−H)⁻.

B. 6-Ethanesulfonyl-N-(4-fluoro-phenyl)-1-oxy-nicotinamide

To 8 mL of trifluoracetic acid at 0° C. was added 1 mL of aqueoushydrogen peroxide (30%) over a 5 minute period followed by the additionof 6-ethylsulfanyl-N-(4-fluoro-phenyl)-nicotinamide (0.050 g, 0.18 mmol)in one portion. The mixture was warmed to room temperature and stirredfor 18 h. Approximately half of the volatiles were removed in vacuo andthe remaining solids diluted with ice water and the solids isolated byvacuum filtration to provide 0.031 g (53%) of the product as a whitesolid. MS (EI) m/z 323.07 (M−H)⁻.

The compounds listed in Table 2 were prepared from6-chloro-N-(4-fluoro-phenyl)-nicotinamide and the appropriate thiolatein the same manner as6-ethanesulfonyl-N-(4-fluoro-phenyl)-1-oxy-nicotinamide: TABLE 2N-(4-Fluoro-phenyl)-6-methanesulfonyl-1-oxy-nicotinamide: MS (EI) m/z309.20 (M − H)⁻.N-(4-Fluoro-phenyl)-1-oxy-6-(propane-2-sulfonyl)-nicotinamide: MS (EI)m/z 337.14 (M − H)⁻.N-(4-Fluoro-phenyl)-1-oxy-6-(propane-1-sulfonyl)-nicotinamide: MS (EI)m/z 337.15 (M − H)⁻.N-(4-Fluoro-phenyl)-1-oxy-6-phenylmethanesulfonyl-nicotinamide: MS (EI)m/z 385.23 (M − H)⁻.6-Cyclopentanesulfonyl-N-(4-fluoro-phenyl)-1-oxy-nicotinamide: MS (EI)m/z 363.20 (M − H)⁻.3-[5-(4-Fluoro-phenylcarbamoyl)-1-oxy-pyridine-2-sulfonyl]-benzoic acid:MS (EI) m/z 415.10 (M − H)⁻.3-[5-(4-Fluoro-phenylcarbamoyl)-1-oxy-pyridine-2-sulfonyl]-propionicacid: MS (EI) m/z 367.17 (M − H)⁻.N-(4-Fluoro-phenyl)-1-oxy-6-(2,3,5,6-tetrafluoro-benzenesulfonyl)-nicotinamide:MS (EI) m/z 443.17 (M − H)⁻.N-(4-Fluoro-phenyl)-1-oxy-6-(toluene-3-sulfonyl)-nicotinamide: MS (EI)m/z 385.29 (M − H)⁻.6-(4-Fluoro-benzenesulfonyl)-N-(4-fluoro-phenyl)-1-oxy-nicotinamide: MS(EI) m/z 389.27 (M − H)⁻.6-Benzenesulfonyl-N-(4-fluoro-phenyl)-1-oxy-nicotinamide: MS (EI) m/z371.18 (M − H)⁻.

Example 21 BIOLOGICAL ACTIVITIES OF REPRESENTATIVE NICOTINALIDE-N-OXIDES

The IL-8 and GRO-α chemokine inhibitory effects of compounds of thepresent invention were determined by the following in vitro assays:

Preparation of PMNs

Peripheral blood from healthy human volunteers was collected intoheparin, diluted in an equal volume of PBS, layered over Ficoll-PaquePlus (Pharmacia Biotech, Uppsala, Sweden), and spun at 400×g for 30 min.The PMN rich fraction was removed and residual erythrocytes were lysedwith hypotonic saline. The polymorphonuclear neutrophils (PMNs) werewashed once with assay buffer (Dulbecco's PBS containing divalentcations and 0.1% endotoxin-free BSA), and resuspended at 1E7 cells/mL inthe same buffer. PMNs were loaded with 5 μM calcein AM (MolecularProbes, Eugene, Oreg.), washed twice and resuspended in assay buffer.

Chemotaxis Assay

Chemotaxis assays with test compounds of the present invention weregenerally performed according to the method described by Frevert et al.,J. Immunol. Meth. 213:41-52 (1998) using either GRO-α or IL-8 assummarized below.

Growth Regulatory Oncogene α (GRO-α) driven chemotaxis assays wereperformed according to the following protocol. The lower chambers of aChemoTx plate (Neuro Probe, Gaithersburg, Md.) were filled with 29 μL of50 nM GRO-α (PeproTech, Rocky Hill, N.J.) and test compound. The emptyupper chambers were affixed to the lower (plate), and 25 μL of PMNsuspension (3E6 cells/mL), without (control) or with 0.04-40 μM testcompound, preincubated 30 min, was added to the upper wells. Testcompounds were dissolved in DMSO (100%) at 20 mM and diluted in assaybuffer to the desired concentrations; final DMSO concentration was 0.2%.Neutrophil migration proceeded for 40 min at 37° C. in a humidifiedincubator with 5% CO₂. After removing nonmigrated cells from the top ofthe plate, migrated cells were quantified by reading fluorescence on aWallac Victor.

Maximum chemotactic response was determined by cells to which nocompound was added (positive control), whereas the negative control(unstimulated) was defined by the absence of chemokine in the lowerchamber. The ratio of the positive to negative controls represents thechemotactic index of the cells. The results from this assay are reportedin Tables 3-5, under the column headings IL-8 and GRO-α, and under thesub-column headings ChTx (for chemotaxis).

Binding Assays

[¹²⁵I] IL-8 (human recombinant) was obtained from NEN Life ScienceProducts, Inc., Boston, Mass., with specific activity of 2200 Ci/mmol.Recombinant human IL-8 was obtained from R&D Systems, Minneapolis, Minn.IL-8 type β receptor membranes were prepared from human Sf9 cellsco-expressed with Gαi3β1γ2 proteins by BioSignal™, Montreal, Canada.Wheat Germ Agglutinin Scintillation Proximity Assay Beads were obtainedfrom Amersham Pharmacia Biotech, Piscataway, N.J. All assays wereperformed in a 96-well, solvent resistant, white PicoPlate obtained fromPackard Instruments. Each reaction mixture contained [¹²⁵I] IL-8 (0.16nM), 5 μg/well IL-8Rβ and 1 mg/well WGA-SPA beads in 25 mM Hepes (pH7.4), containing 2 mM CaCl₂, 1 mM MgCl₂, 0.1% BSA and 0.03% CHAPS. Inaddition, drug or compound of interest was added which had beenpre-dissolved in DMSO so as to reach a final concentration of between0.01 nM and 80 μM, with a maximum final DMSO concentration of 1%.Nonspecific binding was defined by the presence of 8-16 nM unlabelledIL-8. The assay was initiated by the addition of WGA-SPA beads in 25 mMHepes (pH 7.4) containing 2 mM CaCl₂ and 1 mM MgCl₂. After 4-6 hours ofgentle agitation at room temperature, the plate was counted on thePackard TopCount liquid scintillation counter.

Selected compounds were screened by Panlabs (Bothell Wash.) using theirCXCR2 filtermat binding assay. This assay measures binding of[¹²⁵I]Interleukin-8 to human interleukin-CXCR2 (IL8RB) receptors and isalso described in Ahuja, S. K. and Murphy, P. M., J. Biol. Chem.271:20545-20550, 1996. In summary, CHO cells stably transfected with aplasmid encoding the human CXCR2 (IL8RB) chemokine receptor were used toprepare membranes in modified HEPES pH 7.4 buffer. A 2 μg aliquot ofmembrane was incubated with 15 pM [¹²⁵I]Interleukin-8 for 60 minutes atroom temperature. Non-specific binding was estimated in the presence of10 nM interleukin-8. Membranes were filtered and washed 3 times and thefilters were counted to determine [¹²⁵I]Interleukin-8 specificallybound. Compounds were screened at 10 μM and results are reported interms of Specific Binding (%) in Tables 3-5.

Reference Data: Compound IC₅₀ (nM) Ki (nM) nH GROα (or MGSA) 0.098 0.0680.9 Interleukin-8* 0.035 0.024 0.7 NAP-2 3.1 2.2 0.6MGSA = Melanoma Growth Stimulatory Activity;*Indicates standard reference agent used;NAP-2 = Neutrophil Activating Peptide-2

Data obtained by the above-described assays are reported in Tables 3-5.Binding assay results and ChTx (%) results are reported as “*” for %values of less than or equal to 40, and “**” for % values greater than40. When no value appears in a cell, the relevant test was notperformed.

The compounds referred to in Table 3 have the following basic structure,and vary only in the identity of R¹.

The compounds referred to in Table 4 have the following basic structure,and vary only in the identity of R² and R³.

The compounds having test results presented in Table 5 are shown intheir entireties within that Table, with the proviso that PhF representspara-fluorophenyl. TABLE 3 IL-8 GRO-α Binding ChTx R¹ @ 20 uM, % @ 20μM, % Cl— ** (1) **

* *

* *

** *

** **

* *

* **

* **

* *

* *

** **

* **

** **

** **

* *

** **

** **

** **

** **

** **

** *

** *

** *

** **

* ** Br **

* (2) *(1) by the Panlabs assay, this compound also received a rating of “**“.(2) by the Panlabs assay, this compound received a rating of “**”.

TABLE 4 IL-8 GRO-α Binding ChTx —NR²R³ @ 20 uM, % @ 20 μM, %

* **

* **

* **

* **

* **

* **

* *

* **

* *

* *

* **

* **

* **

* **

* **

* **

* *

** **

* *

* *

* *

* *

* *

* *

* **

* **

* **

* **

* **

* **

* **

* **

* C*

* *

* *

* *

* *

* *

* *

* *

**

**

*

**

**

*

*

**

*

**

*

**

**

**

**

**

*

*

**

**

**

**

*

*

*

TABLE 5 GRO-α ChTx Structure @20 μM, %

**

**

*

Example 22 BIOLOGICAL ACTIVITIES OF REPRESENTATIVE NICOTINALIDE-N-OXIDES

Selected N-oxide compounds were tested in the five biological assays asdescribed below, with the results provided in Table 6. In Table 6, “*”indicates a value of less than or equal to 40, while “**” indicates avalue of greater than 40. These biological activities were measured atPanlabs (Bothell, Wash.).

CCR5 Assay (Chemokine CCR5 (Human))

This assay measures the binding of [¹²⁵I]MIP-1β to human chemokine CCR5receptors. See Samson, M., et al., J. Biol. Chem. 272:24934-24941, 1997.In brief, CHO-K1-_(P)242-CCR5 cells stably transfected with a plasmidencoding the human chemokine CCR5 receptor were used to preparemembranes in modified HEPES buffer at pH 7.4 using standard techniques.A 1 μg aliquot wais incubated with 0.1 nM [¹²⁵I]MIP-1β for 120 minutesat 25° C. Non-specific binding was estimated in the presence of 0.1 μMMIP-1β. Membranes were filtered and washed 3 times and filters werecounted to determine [¹²⁵I]MIP-1β specifically bound. Compounds werescreened at 10 μM. The data obtained from this assay is reported inTable 6 (row titled “CCR5”), in terms of specific binding (%).

Reference Data: Compound IC₅₀ (nM) Ki (nM) nH MIP-1α 0.039 0.030 0.8*MIP-1β 0.074 0.057 0.9 RANTES 0.012 0.0093 0.9*Indicates standard reference agent used.MIP = microphage inflammatory protein.RANTES = regulated on activation of normal T-cell expressed and secretedCXCR1 Assay (Chemokine CXCR1/Interleukin IL-8_(A) (Human))

This assay measures binding of [¹²⁵I]Interleukin-8 to humaninterleukin-CXCR1 (ILR8A) receptors. See Ahuja, S. K. and Murphy, P. M.J. Biol. Chem. 271:20545-20550, 1996 for details. In brief, CHO cellsstably transfected with a plasmid encoding the human CXCR1 (IL8RA)chemokine receptor were used to prepare membranes in modified HEPES pH7.4 buffer using standard techniques. A 5 μg aliquot of membrane wasincubated with 8 pM [¹²⁵I]Interleukin-8 for 60 minutes at roomtemperature. Non-specific binding was estimated in the presence of 10 nMinterleukin-8. Membranes were filtered and washed 3 times and thefilters were counted to determine [¹²⁵I]Interleukin-8 specificallybound. Compounds were screened at 10 μM, with the data reported in Table6 (row titled “CXCR1”) in terms of specific binding (%).

Reference Data: Compound IC₅₀ (nM) Ki (nM) nH GROα (or MGSA) >10,000 — —*Interleukin-8 0.035 0.007 1.0 NAP-2 >10,000 — —*Indicates standard reference agent used.GROα = Growth Regulatory Oncogene α.MGSA = Melanoma Growth Stimulatory Activity.NAP-2 = Neutrophil Activating Peptide-2CXCR2 Assay (Chemokine CXCR2/Interleukin IL-8B (Human))

This assay measures binding of [¹²⁵I]Interleukin-8 to humaninterleukin-CXCR2 (IL8RB) receptors. See Ahuja, S. K. and Murphy, P. M.,J. Biol. Chem. 271: 20545-20550, 1996, for details. In brief, CHO cellsstably transfected with a plasmid encoding the human CXCR2 (IL8RB)chemokine receptor were used to prepare membranes in modified HEPES pH7.4 buffer using standard techniques. A 2 μg aliquot of membrane wasincubated with 15 pM [¹²⁵I]Interleukin-8 for 60 minutes at roomtemperature. Non-specific binding was estimated in the presence of 10 nMinterleukin-8. Membranes were filtered and washed 3 times and thefilters were counted to determine [¹²⁵I]Interleukin-8 specificallybound. Compounds were screened at 10 μM, with the results reported inTable 6 (row titled “CXCR2”) in terms of specific binding (%).

Reference Data: Compound IC₅₀ (nM) Ki (nM) nH GROα (or MGSA) 0.098 0.0680.9 *Interleukin-8 0.035 0.024 0.7 NAP-2 3.1 2.2 0.6*Indicates standard reference agent used.GROα = Growth Regulatory Oncogene α.MGSA = Melanoma Growth Stimulatory Activity.NAP-2 = Neutrophil Activating Peptide-2.NPY₁ Assay (Neuropeptide Y₁ (Human))

This assay measures binding of [¹²⁵I]Peptide YY (PYY) to humanneuropeptide Y₁ (NPY₁) receptors. See Fuhlendorff, J., et al. Proc.Natl. Acad. Sci. USA 87:182-186, 1990 and Sheikh, S. P., et al. J. Biol.Chem. 264: 6648-6654, 1989. In brief, SK-N-MC (human neuroblastoma)cells were used in modified HBSS pH 7.4 buffer using standardtechniques. The cells (10⁶) were incubated with 12.5 pM [¹²⁵I]PYY for 45minutes at 25° C. Non-specific binding was estimated in the presence of0.1 μM NPY (human, rat). Cells were centrifuged and pellets were countedto determine [¹²⁵I]PYY specifically bound. Compounds were screened at 10μM, with the results shown in Table 6 (row titled “NPY₁”), reported interms of specific binding (%).

Reference Data: Compound IC₅₀ (nM) Ki (nM) nH *NPY (human, rat) 4.0 3.91.0 NPY (porcine) 7.8 7.6 0.9 NPY (13-36) 2,200 2,100 1.1*Indicates standard reference agent used.Somatostatin Assay

This assay measures binding of [¹²⁵I]tyr¹ Somatostatin to somatostatinreceptors. See Thermos, K. and Reisine, T. Mol. Pharmacol. 33:370-377,1988, and Srikant, C. B. and Heisler, S., Endocrinology 117:271-278,1985. In brief, AtT-20 (mouse pituitary) cells were used to preparemembranes in modified Tris-HCl pH 7.5 buffer using standard techniques.A 200 μg aliquot of membrane was incubated with 0.04 nM [¹²⁵I]tyr¹Somatostatin for 60 minutes at 25° C. Non-specific binding was estimatedin the presence of 1 μM somatostatin-14. Membranes were filtered andwashed 3 times and the filters were counted to determine [¹²⁵I]tyr¹Somatostatin specifically bound. Compounds were screened at 10 μM, withthe data reported in Table 6 (row titled “Somat.”) in terms of specificbinding (%).

Reference Data: Compound IC₅₀ (nM) Ki (nM) nH *Somatostatin 0.93 0.540.8 D-Trp⁸ Somatostatin 0.23 0.13 1.1*Indicates standard reference agent used.

TABLE 6 Structure                    Assay                    

  

         

CCR5 ** * ** CXCR1 ** ** ** * CXCR2 ** ** ** ** NPY₁ * ** * ** Somat. *** * * Structure                    Assay    

   

          

CCR5 ** * ** * * CXCR1 * * * * ** CXCR2 ** ** ** ** ** NPY₁ ** * *Somat. * * * *

All references cited herein are hereby incorporated by reference.

From the foregoing it will be appreciated that, although specificembodiments of the invention have been described herein for purposes ofillustration, various modifications may be made without deviating fromthe spirit and scope of the invention. Accordingly, the invention is notlimited except as by the appended claims.

1. A compound having the structure (I):

and optical isomers, diastereomers, enantiomers and pharmaceuticallyacceptable salts thereof, wherein R¹ is selected from R⁵ andR⁵-(C₁-C₆heteroalkylene)- where R⁵ is selected from hydrogen, halogen,alkyl, heteroalkyl, aryl, heteroaryl, carbocycle aliphatic ring andheterocycle aliphatic ring, amino or hydroxy; R² and R³ areindependently hydrogen, alkyl, heteroalkyl, aryl, aryl(akylene),heteroaryl, heteroaryl(alkylene), carbocycle, carbocycle(alkylene),heterocycle, and heterocycle(alkylene); each occurrence of R⁴ isindependently selected from halogen, alkyl, heteroalkyl, aryl,heteroaryl, carbocycle aliphatic ring and heterocycle aliphatic ring,amino or hydroxy; and n is 0, 1, 2 or
 3. 2. A compound of claim 1wherein n is
 0. 3. A compound of claim 1 wherein n is
 1. 4. A compoundof claim 1 wherein n is 0 or 1 and R² is H.
 5. A compound of claim 4wherein R¹ is R⁵—SO₂— and R⁵ is selected from alkyl, heteroalkyl, aryl,carbocycle, aryl(alkylene), and carbocycle(alkylene).
 6. A compound ofclaim 5 wherein, for R⁵, alkyl is C₁-C₁₀alkyl; heteroalkyl isC₁-C₁₀alkyl with 1, 2 or 3 heteroatoms selected from N, O and S; aryl isphenyl, substituted phenyl, naphthyl or substituted naphthyl; carbocycleis C₃-C₈carbocycle; and alkylene is C₁-C₁₀alkylene.
 7. A compound ofclaim 5 wherein R¹ is selected from (C₁-C₆alkyl)SO₂—, PhSO₂—,fluorinatedphenylSO₂—, PhCH₂SO₂—, cyclopentylSO₂—, m-carboxyphenylSO₂—,m-methylphenylSO₂—, and HOOC—(C₁-C₄alkylene)SO₂—.
 8. A compound of claim1 wherein R¹ is selected from halogen, amino, hydrocarbylamino,dihydrocarbylamino, hydrocarbyloxy, hydrocarbylthio, heterocyclyl,(heteroalkyl)amino, and (heteroaryl)amino.
 9. A compound of claim 7wherein R¹ is selected from amino, (C₁-C₆alkyl)(C₁-C₆alkyl)amino, PhNH—,PhCH₂NH—,

and HOCH₂CH₂NH—.
 10. A compound of claim 8 wherein R¹ is selected fromhalide and (C₁-C₆alkyl)S—.
 11. A compound of claim 10 wherein R¹ ischloride.
 12. A compound of claim 4 wherein R³ is selected from aryl,aryl(alkylene), heteroaryl, and heteroaryl(alkylene).
 13. A compound ofclaim 12 wherein R³ is aryl.
 14. A compound of claim 1 having structure(II)


15. A compound of claim 14 wherein R¹ is selected from (C₁₋₆alkyl)SO₂—,PhSO₂—, fluorinatedphenylSO₂—, PhCH₂SO₂—, cyclopentylSO₂—,m-carboxyphenylSO₂—, m-methylphenylSO₂—, and HOOC—(C₁-C₄alkylene)SO₂—.16. A compound of claim 4 wherein R³ is benzyl or phenyl, the benzyl orphenyl having 0, 1, 2, 3 or 4 substituents selected from alkoxy,alkoxycarbonyl, alkyl, alkylamido, alkylcarbonyl, amido, benzyloptionally substituted with halogen, benzyloxy, carboxy, cyano,dialkylamido, haloalkyl, haloalkyloxy, halogen, hydroxy, nitro,oxoalkyl, phenyl optionally substituted with halogen, thioalkyl,thiocyanate, and thiohaloalkyl.
 17. A compound of claim 1 wherein R³ isselected from cycloalkyl, cycloalkyl(alkylene),cycloalkyl(heteroalkylene), heterocycloalkyl,heterocycloalkyl(alkylene), heterocycloalkyl(heteroalkylene),heteroaryl, heteroaryl(alkylene), and heteroaryl(heteroalkylene).
 18. Acompound of claim 1 wherein said compound is6-Chloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide.
 19. A compound of claim1 wherein said compound isN-(4-Fluoro-phenyl)-6-(2-hydroxy-ethylamino)-1-oxy-nicotinamide.
 20. Acompound of claim 1 wherein said compound is6-Bromo-N-(4-fluoro-phenyl)-1-oxy-nicotinamide.
 21. A compound of claim1 wherein said compound is5,6-Dichloro-N-(4-fluoro-phenyl)-1-oxy-nicotinamide.
 22. A compound ofclaim 1 wherein said compound is6-Ethanesulfonyl-N-(4-fluoro-phenyl)-1-oxy-nicotinamide.
 23. A compoundof claim 1 wherein said compound isN-(4-Fluoro-phenyl)-1-oxy-6-(propane-2-sulfonyl)-nicotinamide.
 24. Acompound of claim 1 wherein said compound isN-(4-Fluoro-phenyl)-6-methanesulfonyl-1-oxy-nicotinamide.
 25. A compoundof claim 1 wherein said compound is6-Benzenesulfonyl-N-(4-fluoro-phenyl)-1-oxy-nicotinamide.
 26. A compoundof claim 1 wherein said compound isN-(4-Fluoro-phenyl)-1-oxy-6-phenylmethanesulfonyl-nicotinamide.
 27. Acompound of claim 1 wherein said compound is6-Chloro-N-(3-chloro-4-fluoro-phenyl)-1-oxy-nicotinamide.
 28. A compoundof claim 1 wherein said compound is6-Chloro-N-(4-iodo-phenyl)-1-oxy-nicotinamide.
 29. A compound of claim 1wherein R¹ is selected from halogen, heteroalkyl or amino, R² is H, R³is aryl and R⁴ is H.
 30. A composition comprising a compound of claim 1and a pharmaceutically acceptable carrier, adjuvant or incipient.
 31. Amethod for antagonizing chemokine receptors comprising administering toa patient in need thereof an effective amount of a compound of claim 1.32. A method for inhibiting a chemokine-mediated cellular eventcomprising administering to a patient in need thereof an effectiveamount of a compound of claim
 1. 33. A method of claim 32 wherein thecompound inhibits IL-8 and or GRO-α driven neutrophil chemotaxis. 34.The method of claim 32 wherein the compound inhibits a CXCR1 receptor.35. The method of claim 32 wherein the compound inhibits a CXCR2receptor.
 36. The method of claim 32 for the treatment of a disorderselected from Inflammatory Bowel Disease (IBD), psoriasis, rheumatoidarthritis, Acute Respiratory Distress Syndrome (ARDS), cancer,atherosclerosis, reperfusion injury, and graft vs. host disease.
 37. Amethod for inhibiting a G-protein-coupled, seven-transmembrane domain(7TM) receptor in a patient comprising administering to the patient acompound of claim 1 in an amount effective to inhibit the receptor. 38.A method of claim 37 wherein the compound modulates the binding ofPeptide YY (PYY) to a NPY cell receptor.
 39. A method of claim 37wherein the compound modulates the binding of somatostatin to asomatostatin cell receptor.
 40. A method of claim 37 wherein thecompound modulates the binding of MIP-1β to a CCR5 cell receptor.
 41. Amethod for treating an inflammation event, comprising administering to apatient in need thereof, through a therapeutically or prophylacticallyacceptable manner, a therapeutically or pharmaceutically effectiveamount of the compound of claim
 1. 42. The method of claim 41 whereinadministration is selected from transdermal, oral, intravenous,intramuscular, vaginal, rectal, pulmonary, subcutaneous, sublingual andtransmucosal administration.
 43. A method for identifying a bindingpartner to a compound of claim 1 comprising: immoblizing proteins knownto be involved in the TNF-α signaling pathway onto a suitable carrier;and passing a solution of said compounds in isolation or mixture oversaid proteins and analyzing for compound:protein complex formation usingsurface plasmon resonance (SPR).
 44. A method for identifying a bindingpartner to a compound of claim 1 comprising: providing said compound(s)bound to a solid support to provide solid phase compounds; contacting acell or cell components with said solid phase compounds in isolation ormixture; removing uncomplexed cellular material, for example by gentlewashing with aqueous buffer; and recovering said binding partner fromthe solid phase compounds.